Immune modulatory effects of lenalidomide on the cultured peripheral blood mononuclear cells from vitiligo patients
| العنوان: | Immune modulatory effects of lenalidomide on the cultured peripheral blood mononuclear cells from vitiligo patients |
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| المؤلفون: | Naveed Pervaiz, Harjot Kaur, Ravinder Kumar, Davinder Parsad, Dr. Ravinder Kumar |
| المصدر: | Dermatologic Therapy. 33 |
| بيانات النشر: | Hindawi Limited, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | T cell, Vitiligo, Dermatology, CD8-Positive T-Lymphocytes, Peripheral blood mononuclear cell, Interferon-gamma, 030207 dermatology & venereal diseases, 03 medical and health sciences, 0302 clinical medicine, Immune system, medicine, Humans, Lenalidomide, Autoimmune disease, Tumor Necrosis Factor-alpha, business.industry, General Medicine, medicine.disease, medicine.anatomical_structure, 030220 oncology & carcinogenesis, Immunology, Leukocytes, Mononuclear, Tumor necrosis factor alpha, business, CD8, medicine.drug |
| الوصف: | Vitiligo is a depigmentary disease in which epidermal melanocytes are lost. It is considered to be an autoimmune disease. Lenalidomide, an immunomodulatory drug is being employed in the treatment of various autoimmune and inflammatory disorders. In the present manuscript, the effect of lenalidomide on T cells and major cytokines in the cultured peripheral blood mononuclear cells (PBMCs) derived from vitiligo patients was checked. Eight patients with a clinical diagnosis of active vitiligo volunteered for the study. Blood was collected from them and PBMCs were isolated, cultured, and treated with lenalidomide. After 72 hours, PBMCs were harvested and checked for CD8+ and CD4+ T cells by flow cytometry. Further supernatant was collected and the levels of cytokines namely tumor necrosis factor-alpha (TNF-α), interferon-gamma (IFN-γ), interleukin-10 (IL-10), and interleukin-4 (IL-4) were checked using ELISA kits. Lenalidomide nonsignificantly decreased the level of CD8+ T cells but increased CD4+ T cells leading to increased CD4+ /CD8+ T cell ratio. It declined the level of pro-inflammatory cytokines, that is, TNF-α and IFN-γ whereas elevated anti-inflammatory cytokines, that is, IL-10 and IL-4, thus ultimately decreasing the ratio of pro-inflammatory to anti-inflammatory cytokines. Lenalidomide suppressed the proliferation of T lymphocytes and modulated the cytokines secretion toward an anti-inflammatory profile. |
| تدمد: | 1529-8019 1396-0296 |
| DOI: | 10.1111/dth.14473 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cffc7c0c989cf94197de6b9444996331 https://doi.org/10.1111/dth.14473 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....cffc7c0c989cf94197de6b9444996331 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15298019 13960296 |
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| DOI: | 10.1111/dth.14473 |