Culturing periprosthetic tissue in BacT/Alert® Virtuo blood culture system leads to improved and faster detection of prosthetic joint infections
| العنوان: | Culturing periprosthetic tissue in BacT/Alert® Virtuo blood culture system leads to improved and faster detection of prosthetic joint infections |
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| المؤلفون: | Merethe E. O. Røkeberg, Anne-Merethe Hanssen, Gunnar Skov Simonsen, Johanna U. Ericson Sollid, Adriana Sanabria, Mona Johannessen |
| المصدر: | BMC Infectious Diseases, Vol 19, Iss 1, Pp 1-8 (2019) BMC Infectious Diseases |
| بيانات النشر: | BMC, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, Prosthetic joint infection, Microbiological culture, Prosthesis-Related Infections, Time Factors, BacT/Alert® Virtuo blood culture system, Prosthetic joint, Joint Prosthesis, 030106 microbiology, Periprosthetic, Sensitivity and Specificity, Microbiology, Specimen Handling, lcsh:Infectious and parasitic diseases, 03 medical and health sciences, 0302 clinical medicine, medicine, Humans, Blood culture, lcsh:RC109-216, 030212 general & internal medicine, Time to detection, medicine.diagnostic_test, Bacteria, business.industry, Bact alert, VDP::Medical disciplines: 700::Basic medical, dental and veterinary science disciplines: 710::Medical microbiology: 715, Periprosthetic tissue specimens, Blood culture bottle, Infectious Diseases, Blood culture bottles, Blood Culture, VDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710::Medisinsk mikrobiologi: 715, business, Research Article |
| الوصف: | Background Blood culture bottles (BCBs) provide a semiautomated method for culturing periprosthetic tissue specimens. A study evaluating BCBs for culturing clinical samples other than body fluids is needed before implementation into clinical practice. Our objective was to evaluate use of the BacT/Alert® Virtuo blood culture system for culturing periprosthetic tissue specimens. Methods The study was performed through the analysis of spiked (n = 36) and clinical (n = 158) periprosthetic tissue samples. Clinical samples were analyzed by the BCB method and the results were compared to the conventional microbiological culture-based method for time to detection and microorganisms identified. Results The BacT/Alert® Virtuo blood culture system detected relevant bacteria for prosthetic joint infection in both spiked and clinical samples. The BCB method was found to be as sensitive (79%) as the conventional method (76%) (p = 0.844) during the analyses of clinical samples. The BCB method yielded positive results much faster than the conventional method: 89% against 27% detection within 24 h, respectively. The median detection time was 11.1 h for the BCB method (12 h and 11 h for the aerobic and the anaerobic BCBs, correspondingly). Conclusion We recommend using the BacT/Alert® Virtuo blood culture system for analyzing prosthetic joint tissue, since this detect efficiently and more rapidly a wider range of bacteria than the conventional microbiological method. Electronic supplementary material The online version of this article (10.1186/s12879-019-4206-x) contains supplementary material, which is available to authorized users. |
| اللغة: | English |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cf4f77a7e02fc1af4380399a5c7b5b52 https://hdl.handle.net/10037/16037 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....cf4f77a7e02fc1af4380399a5c7b5b52 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |