Cholesterol: A modulator of the phagocyte NADPH oxidase activity - A cell-free study
| العنوان: | Cholesterol: A modulator of the phagocyte NADPH oxidase activity - A cell-free study |
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| المؤلفون: | Tania Bizouarn, Rawand Masoud, Chantal Houée-Levin |
| المصدر: | Redox Biology Redox Biology, Vol 3, Iss C, Pp 16-24 (2014) |
| بيانات النشر: | The Authors. Published by Elsevier B.V. |
| مصطلحات موضوعية: | PMSF, phenylmethanesulfonyl fluoride, Enzyme complex, Neutrophils, Clinical Biochemistry, AA, arachidonic acid, Biochemistry, LDL, low density lipoprotein, chemistry.chemical_compound, Superoxide production, Cell-free system, MβCD, methyl-β-cyclodextrin, NADPH, reduced β-nicotinamide adenine dinucleotide phosphate, Superoxides, DTT, dithiotreitol, lcsh:QH301-705.5, PtdIns(3)P, phosphatidyl-inositol3-phosphate, Lipid raft, chemistry.chemical_classification, lcsh:R5-920, Phagocytes, Arachidonic Acid, FMLP, formyl-methionyl-leucyl-phenylalanine, PX, phox homology domain, NADPH oxidase, biology, Superoxide, beta-Cyclodextrins, Tryptophan, IPTG, isopropylthiogalatoside, Arachidonic acid activation, FAD, flavin adenine dinucleotide, LR, lipid raft, Cholesterol, Membrane part, lcsh:Medicine (General), Cyt b558, cytochrome b558, Research Paper, HEPES, [4-(2-hydroxyethyl)piperazine-1-yl]ethanesulfonic acid, LB, Luria Bertoni, Enzyme activator, PBS, phosphate buffer saline, ROS, reactive oxygen species, MF, membrane fractions, Animals, Humans, Horses, GTP, guanosine-5′-triphosphate, Reactive oxygen species, EDTA, ethylenediaminetetraacetic acid, Organic Chemistry, Cytc, cytochrome c, NADPH Oxidases, PtdIns(3,4)P2, phosphatidylinositol(3,4)-bisphosphate, Enzyme Activation, Kinetics, Enzyme, lcsh:Biology (General), chemistry, SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis, biology.protein |
| الوصف: | The NADPH oxidase Nox2, a multi-subunit enzyme complex comprising membrane and cytosolic proteins, catalyzes a very intense production of superoxide ions O2•−, which are transformed into other reactive oxygen species (ROS). In vitro, it has to be activated by addition of amphiphiles like arachidonic acid (AA). It has been shown that the membrane part of phagocyte NADPH oxidase is present in lipid rafts rich in cholesterol. Cholesterol plays a significant role in the development of cardio-vascular diseases that are always accompanied by oxidative stress. Our aim was to investigate the influence of cholesterol on the activation process of NADPH oxidase. Our results clearly show that, in a cell-free system, cholesterol is not an efficient activator of NADPH oxidase like arachidonic acid (AA), however it triggers a basal low superoxide production at concentrations similar to what found in neutrophile. A higher concentration, if present during the assembly process of the enzyme, has an inhibitory role on the production of O2•−. Added cholesterol acts on both cytosolic and membrane components, leading to imperfect assembly and decreasing the affinity of cytosolic subunits to the membrane ones. Added to the cytosolic proteins, it retains their conformations but still allows some conformational change induced by AA addition, indispensable to activation of NADPH oxidase. Highlights • Natural cholesterol is important for the NADPH oxidase function. • Added cholesterol alone activates slightly the NADPH oxidase. • Cholesterol addition lowers the AA dependent activity of NADPH oxidase. • Added cholesterol acts on both cytosolic and membrane components. |
| اللغة: | English |
| تدمد: | 2213-2317 |
| DOI: | 10.1016/j.redox.2014.10.001 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ceeb8c69bd9cbf977c4b03acba0f3c96 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....ceeb8c69bd9cbf977c4b03acba0f3c96 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 22132317 |
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| DOI: | 10.1016/j.redox.2014.10.001 |