Extenders modify the seminal plasma ability to minimize freeze‐thaw damage on ram sperm
| العنوان: | Extenders modify the seminal plasma ability to minimize freeze‐thaw damage on ram sperm |
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| المؤلفون: | Adriana Cano, Rafael Ramírez-Vasquez, F. Hozbor, Andreina Cesari, Micaela B Greco |
| المصدر: | Reproduction in Domestic Animals. 54:1621-1629 |
| بيانات النشر: | Wiley, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Male, food.ingredient, Cryopreservation, law.invention, Andrology, 03 medical and health sciences, Cryoprotective Agents, 0302 clinical medicine, Endocrinology, food, law, Yolk, Freezing, Lecithins, medicine, Animals, Cell damage, Sperm motility, Sperm plasma membrane, Sheep, 030219 obstetrics & reproductive medicine, urogenital system, Chemistry, Extender, 0402 animal and dairy science, 04 agricultural and veterinary sciences, medicine.disease, Egg Yolk, Spermatozoa, 040201 dairy & animal science, Sperm, Sperm Motility, Animal Science and Zoology, Soybeans, Percoll, Semen Preservation, Biotechnology |
| الوصف: | Seminal plasma (SP) proteins interact with sperm plasma membrane (PM) modulating its functionality. It has been shown that SP proteins can reverse the damage caused by freeze-thaw; however in these studies, SP has been added to washed sperm (i.e., cells depleted from homologous SP and extender). The aim of the current study was to assess whether the egg yolk-based extender (EY) modifies SP ability to ameliorate sperm parameters in frozen-thawed ram spermatozoa. Ejaculates were diluted in EY or soybean lecithin-based extender (SL) and evaluated before and after freezing to measure the cell damage according to the extender. Even when all classical parameters decreased after freezing, as expected (p < .05), there was no effect of the extender. SP treatment was applied after freeze-thaw. Sperm were incubated with SP (20% v/v) in the presence of either EY or SL, and sperm parameters were assessed after thawing compared with the same treatments after Percoll sperm selection (washed). Treatments with 20% SP improved sperm total and progressive motility compared with controls regardless of washing and extender (p < .05); however, washed sperm showed higher percentage of total sperm motility compared with those unwashed (p < .05). Moreover, treatment with 20% SP showed significantly higher percentages of PM integrity, sperm with intact acrosomes, integrity of chromatin and non-capacitated sperm in samples diluted with EY when washed before treatment compared with the other conditions (p < .05). It was concluded that the presence of the extenders and particularly egg yolk alters the SP capacity to reduce the cryodamage. |
| تدمد: | 1439-0531 0936-6768 |
| DOI: | 10.1111/rda.13571 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ce361dbb030081cac9943be5eb9a1d94 https://doi.org/10.1111/rda.13571 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....ce361dbb030081cac9943be5eb9a1d94 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14390531 09366768 |
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| DOI: | 10.1111/rda.13571 |