Proteins and RNA in mouse L cell core nucleoli and nucleolar matrix
| العنوان: | Proteins and RNA in mouse L cell core nucleoli and nucleolar matrix |
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| المؤلفون: | Tri Van Nguyen, Robert I. Bolla, James C. Powers, David Schlessinger, Yukio Shiomi |
| المصدر: | Biochemistry. 25:5745-5751 |
| بيانات النشر: | American Chemical Society (ACS), 1986. |
| سنة النشر: | 1986 |
| مصطلحات موضوعية: | Nucleolus, Biology, Biochemistry, Cell Line, Mice, chemistry.chemical_compound, Cricetulus, L Cells, Liver Neoplasms, Experimental, Ribosomal protein, Cricetinae, Animals, Genetics, Ovary, Leupeptin, RNA, Ribosomal RNA, Rats, Nucleoprotein, Molecular Weight, Nucleoproteins, chemistry, Electrophoresis, Polyacrylamide Gel, Female, Cell Nucleolus, Lamin, Small nuclear RNA, HeLa Cells |
| الوصف: | When intact nucleoli were prepared in the presence of enough leupeptin and phenylmethanesulfonyl fluoride to inhibit protease action, electrophoretic patterns of their constituent proteins were reproducible and very similar for L, HeLa, CHO, and rat hepatoma cells. "Core nucleoli", defined as that nucleolar fraction which remains after extensive DNase I action, had a protein composition similar to that of crude intact nucleoli, but were enriched for snRNA U3. Core nucleolar proteins included all of the histones, ribosomal proteins, and phosphorylated proteins with mobilities corresponding to 110 (protein C23) and 160 kilodaltons (kDa). The presence of protein C23 and of lamins A and C in nucleoli and core nucleoli was further verified by reaction with specific antibodies after one- or two-dimensional electrophoresis. A class of higher molecular weight proteins, ranging from 70 to greater than 200 kDa by mobility, was observed. It included at least 25 specific proteins, almost all of them highly acidic (pI less than 3.5). Treatment of core nucleoli with ethylenediaminetetraacetic acid/hypotonic buffer solubilized 30-35% of the small and large molecular weight proteins. In contrast, washing core nucleoli with 2 M NaCl selectively released U3 snRNA, 95% of the ribosomal RNA, and about half of the proteins, including C23 and most of the histones, ribosomal proteins, and other lower molecular weight proteins. The fraction remaining insoluble, "nucleolar matrix", was enriched for proteins of 34 and 57 kDa, lamins A and C, and most higher molecular weight proteins, as well as a portion of ribosomal spacer DNA. |
| تدمد: | 1520-4995 0006-2960 |
| DOI: | 10.1021/bi00367a059 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cdb75f54bf1abbcaaa5a6bb670942acd https://doi.org/10.1021/bi00367a059 |
| رقم الانضمام: | edsair.doi.dedup.....cdb75f54bf1abbcaaa5a6bb670942acd |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15204995 00062960 |
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| DOI: | 10.1021/bi00367a059 |