Feasibility and clinical utility of comprehensive genomic profiling of hematological malignancies
| العنوان: | Feasibility and clinical utility of comprehensive genomic profiling of hematological malignancies |
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| المؤلفون: | Suguru Fukuhara, Yuji Oshikawa‐Kumade, Yasunori Kogure, Sumito Shingaki, Hirokazu Kariyazono, Yoshiya Kikukawa, Junji Koya, Yuki Saito, Mariko Tabata, Kota Yoshifuji, Kota Mizuno, Akiko Miyagi‐Maeshima, Hiromichi Matsushita, Masanaka Sugiyama, Chitose Ogawa, Yoshihiro Inamoto, Takahiro Fukuda, Masato Sugano, Nobuhiko Yamauchi, Yosuke Minami, Makoto Hirata, Teruhiko Yoshida, Takashi Kohno, Shinji Kohsaka, Hiroyuki Mano, Yuichi Shiraishi, Seishi Ogawa, Koji Izutsu, Keisuke Kataoka |
| المصدر: | Cancer Science. 113:2763-2777 |
| بيانات النشر: | Wiley, 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Cancer Research, Oncology, Hematologic Neoplasms, Mutation, Feasibility Studies, High-Throughput Nucleotide Sequencing, Humans, Genomics, Prospective Studies, General Medicine |
| الوصف: | Identification of genetic alterations through next-generation sequencing (NGS) can guide treatment decision-making by providing information on diagnosis, therapy selection, and prognostic stratification in patients with hematological malignancies. Although the utility of NGS-based genomic profiling assays was investigated in hematological malignancies, no assays sufficiently cover driver mutations, including recently discovered ones, as well as fusions and/or pathogenic germline variants. To address these issues, here we have devised an integrated DNA/RNA profiling assay to detect various types of somatic alterations and germline variants at once. Particularly, our assay can successfully identify copy number alterations and structural variations, including immunoglobulin heavy chain translocations, IKZF1 intragenic deletions, and rare fusions. Using this assay, we conducted a prospective study to investigate the feasibility and clinical usefulness of comprehensive genomic profiling for 452 recurrently altered genes in hematological malignancies. In total, 176 patients (with 188 specimens) were analyzed, in which at least one alteration was detected in 171 (97%) patients, with a median number of total alterations of 7 (0-55). Among them, 145 (82%), 86 (49%), and 102 (58%) patients harbored at least one clinically relevant alteration for diagnosis, treatment, and prognosis, respectively. The proportion of patients with clinically relevant alterations was the highest in acute myeloid leukemia, whereas this assay was less informative in T/natural killer-cell lymphoma. These results suggest the clinical utility of NGS-based genomic profiling, particularly for their diagnosis and prognostic prediction, thereby highlighting the promise of precision medicine in hematological malignancies. |
| تدمد: | 1349-7006 1347-9032 |
| DOI: | 10.1111/cas.15427 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cd810ca586cb7ec76e0891b5d7979460 https://doi.org/10.1111/cas.15427 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....cd810ca586cb7ec76e0891b5d7979460 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13497006 13479032 |
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| DOI: | 10.1111/cas.15427 |