Single-cell stabilization method identifies gonadotrope transcriptional dynamics and pituitary cell type heterogeneity
| العنوان: | Single-cell stabilization method identifies gonadotrope transcriptional dynamics and pituitary cell type heterogeneity |
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| المؤلفون: | Hanna Pincas, Nadejda M. Tsankova, Yongchao Ge, Stuart C. Sealfon, Peter Smibert, Harold Swerdlow, Jessica Tome-Garcia, Venugopalan D. Nair, Gregory R. Smith, Boris M. Hartmann, Michel Zamojski, Miguel Fribourg, Daniel J. Bernard, Chirine Toufaily, Marlon Stoeckius, Frederique Ruf-Zamojski, William Stephenson, Judith L Turgeon |
| المصدر: | Ruf-Zamojski, Frederique; Ge, Yongchao; Nair, Venugopalan; Zamojski, Michel; Pincas, Hanna; Toufaily, Chirine; et al.(2018). Single-cell stabilization method identifies gonadotrope transcriptional dynamics and pituitary cell type heterogeneity.. Nucleic acids research, 46(21), 11370-11380. doi: 10.1093/nar/gky991. UC Davis: Retrieved from: http://www.escholarship.org/uc/item/273146m1 Nucleic Acids Research |
| بيانات النشر: | Oxford University Press (OUP), 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | Transcriptional Activation, 0301 basic medicine, RNA Stability, Cell, Gonadotrophs, Gonadotropin-releasing hormone, Buffers, Biology, Gonadotropic cell, Gonadotropin-Releasing Hormone, Transcriptome, Genetic Heterogeneity, Mice, 03 medical and health sciences, Transcription (biology), Cell Line, Tumor, Information and Computing Sciences, Genetics, medicine, Animals, RNA, Messenger, Genes, Immediate-Early, Early Growth Response Protein 2, Early Growth Response Protein 1, Regulation of gene expression, Dose-Response Relationship, Drug, Sequence Analysis, RNA, RNA, Genomics, Biological Sciences, Cell biology, 030104 developmental biology, medicine.anatomical_structure, Cell culture, Single-Cell Analysis, Proto-Oncogene Proteins c-fos, Environmental Sciences, Developmental Biology |
| الوصف: | Immediate-early response genes (IEGs) are rapidly and transiently induced following an extracellular signal. Elucidating the IEG response patterns in single cells (SCs) requires assaying large numbers of timed samples at high accuracy while minimizing handling effects. To achieve this, we developed and validated RNA stabilization Buffer for Examination of Single-cell Transcriptomes (RNA-Best), a versatile single-step cell and tissue preservation protocol that stabilizes RNA in intact SCs without perturbing transcription patterns. We characterize for the first time SC heterogeneity in IEG responses to pulsatile gonadotropin-releasing hormone (GnRH) stimuli in pituitary gonadotrope cells. Our study identifies a gene-specific hierarchical pattern of all-or-none transcript induction elicited by increasing concentrations of GnRH. This quantal pattern of gene activation raises the possibility that IEG activation, when accurately resolved at the SC level, may be mediated by gene bits that behave as pure binary switches. |
| وصف الملف: | application/pdf |
| تدمد: | 1362-4962 0305-1048 |
| DOI: | 10.1093/nar/gky991 |
| DOI: | 10.1093/nar/gky991. |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cd3fa7c20459fe142498d9fa3e7c3beb https://doi.org/10.1093/nar/gky991 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....cd3fa7c20459fe142498d9fa3e7c3beb |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13624962 03051048 |
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| DOI: | 10.1093/nar/gky991 |