التفاصيل البيبلوغرافية
| العنوان: |
Insight into Structure and Assembly of the Nuclear Pore Complex by Utilizing the Genome of a Eukaryotic Thermophile |
| المؤلفون: |
Manimozhiyan Arumugam, Phillip Sarges, Dirk Flemming, Vera van Noort, Ed Hurt, Stefan Amlacher, Damien P. Devos, Peer Bork, Ruth Kunze |
| المصدر: |
Cell. 146(2):277-289 |
| بيانات النشر: |
Elsevier BV, 2011. |
| سنة النشر: |
2011 |
| مصطلحات موضوعية: |
Genetics, Biochemistry, Genetics and Molecular Biology(all), Molecular Sequence Data, Sequence alignment, Computational biology, Chaetomium, Biology, biology.organism_classification, Genome, General Biochemistry, Genetics and Molecular Biology, Molecular machine, Fungal Proteins, Nuclear Pore Complex Proteins, Chaetomium thermophilum, Two-Hybrid System Techniques, Nuclear Pore, Short linear motif, Eukaryote, Amino Acid Sequence, Nucleoporin, Genome, Fungal, Nuclear pore, Sequence Alignment, Phylogeny |
| الوصف: |
SummaryDespite decades of research, the structure and assembly of the nuclear pore complex (NPC), which is composed of ∼30 nucleoporins (Nups), remain elusive. Here, we report the genome of the thermophilic fungus Chaetomium thermophilum (ct) and identify the complete repertoire of Nups therein. The thermophilic proteins show improved properties for structural and biochemical studies compared to their mesophilic counterparts, and purified ctNups enabled the reconstitution of the inner pore ring module that spans the width of the NPC from the anchoring membrane to the central transport channel. This module is composed of two large Nups, Nup192 and Nup170, which are flexibly bridged by short linear motifs made up of linker Nups, Nic96 and Nup53. This assembly illustrates how Nup interactions can generate structural plasticity within the NPC scaffold. Our findings therefore demonstrate the utility of the genome of a thermophilic eukaryote for studying complex molecular machines. |
| تدمد: |
0092-8674 |
| DOI: |
10.1016/j.cell.2011.06.039 |
| URL الوصول: |
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cc9b82d8ebfafb787465acafd50bc9e6 |
| Rights: |
OPEN |
| رقم الانضمام: |
edsair.doi.dedup.....cc9b82d8ebfafb787465acafd50bc9e6 |
| قاعدة البيانات: |
OpenAIRE |