CkP1 bacteriophage, a S16-like myovirus that recognizes Citrobacter koseri lipopolysaccharide through its long tail fibers
| العنوان: | CkP1 bacteriophage, a S16-like myovirus that recognizes Citrobacter koseri lipopolysaccharide through its long tail fibers |
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| المؤلفون: | Hugo Oliveira, Sílvio Santos, Diana P. Pires, Dimitri Boeckaerts, Graça Pinto, Rita Domingues, Jennifer Otero, Yves Briers, Rob Lavigne, Mathias Schmelcher, Andreas Dötsch, Joana Azeredo |
| المساهمون: | Universidade do Minho |
| المصدر: | Applied Microbiology and Biotechnology, 107 (11) |
| بيانات النشر: | Springer Nature, 2023. |
| سنة النشر: | 2023 |
| مصطلحات موضوعية: | Citrobacter, Bacterial infection, Bacteriophage, Long tail fiber, Control, Diagnostics, Science & Technology, General Medicine, Applied Microbiology and Biotechnology, Biotechnology |
| الوصف: | The online version contains supplementary material available at https://doi.org/10.1007/s00253-023-12547-8. Citrobacter koseri is an emerging Gram-negative bacterial pathogen, which causes urinary tract infections. We isolated and characterized a novel S16-like myovirus CKP1 (vB\_CkoM\\_CkP1), infecting C. koseri. CkP1 has a host range covering the whole C. koseri species, i.e., all strains that were tested, but does not infect other species. Its linear 168,463-bp genome contains 291 coding sequences, sharing sequence similarity with the Salmonella phage S16. Based on surface plasmon resonance and recombinant green florescence protein fusions, the tail fiber (gp267) was shown to decorate C. koseri cells, binding with a nanomolar affinity, without the need of accessory proteins. Both phage and the tail fiber specifically bind to bacterial cells by the lipopolysaccharide polymer. We further demonstrate that CkP1 is highly stable towards different environmental conditions of pH and temperatures and is able to control C. koseri cells in urine samples. Altogether, CkP1 features optimal in vitro characteristics to be used both as a control and detection agent towards drug-resistant C. koseri infections. Open access funding provided by FCT|FCCN (b-on). This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UIDB/04469/2020 unit. DB is supported by the Research Foundation – Flanders (FWO), grant number 1S69520N. JO received a predoctoral fellowship from the UAB and a FEMS research and training grant. info:eu-repo/semantics/publishedVersion |
| وصف الملف: | application/pdf; application/application/pdf |
| اللغة: | English |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cc5bbb3152dc2942cb3dc09e3ae2f1b7 https://hdl.handle.net/1822/84652 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....cc5bbb3152dc2942cb3dc09e3ae2f1b7 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |