التفاصيل البيبلوغرافية
| العنوان: |
Interaction with the IQ3 motif of myosin-10 is required for calmodulin-like protein-dependent filopodial extension |
| المؤلفون: |
Richard D. Bennett, Amy S. Mauer, Emanuel E. Strehler, Ariel J. Caride |
| المصدر: |
FEBS Letters. (16):2377-2381 |
| بيانات النشر: |
Federation of European Biochemical Societies. Published by Elsevier B.V. |
| مصطلحات موضوعية: |
Calmodulin, Phenylalanine, Amino Acid Motifs, Molecular Sequence Data, Biophysics, Motility, Video microscopy, Myosins, Biochemistry, Structural Biology, Myosin, Genetics, Humans, Protein Interaction Domains and Motifs, Amino Acid Sequence, Pseudopodia, Binding site, Molecular Biology, Filopodia, Binding Sites, IQ domain, biology, Chemistry, Cell Biology, bacterial infections and mycoses, Myosin-10, Molecular biology, Calmodulin-like protein, Cell biology, Transport protein, Protein Transport, stomatognathic diseases, Amino Acid Substitution, biology.protein, HeLa Cells |
| الوصف: |
Calmodulin-like protein (CLP) is a specific light chain of unconventional myosin-10 (Myo10) and enhances Myo10-dependent filopodial extension. Here we show that phenylalanine-795 in the third IQ domain (IQ3) of Myo10 is critical for CLP binding. Remarkably, mutation of F795 to alanine had little effect on calmodulin binding to IQ3. Fluorescence microscopy and time-lapse video microscopy showed that HeLa cells expressing CLP and transiently transfected with GFP-Myo10-F795A exhibited significantly shorter filopodia and decreased intrafilopodial motility compared to wildtype GFP-Myo10-transfected cells. Thus, F795 represents a unique anchor for CLP and is essential for CLP-mediated Myo10 function in filopodial extension and motility.Structured summaryMINT-6595901: GST-IQ3 Myo10 (uniprotkb:Q9HD67) binds (MI:0407) to CLP (uniprotkb:P27482) by pull down (MI:0096)MINT-6596000: CLP (uniprotkb:P27482) and GST-IQ3 Myo10 (uniprotkb:Q9HD67) bind (MI:0407) by classical fluorescence spectroscopy (MI:0017)MINT-6596013: CaM (uniprotkb:P62158) and GST-IQ3 Myo10 (uniprotkb:Q9HD67) bind (MI:0407) by classical fluorescence spectroscopy (MI:0017)MINT-6595938:GST-IQ3 Myo10 (uniprotkb:Q9HD67) binds (MI:0407) to CaM (uniprotkb:P62158) by pull down (MI:0096) |
| اللغة: |
English |
| تدمد: |
0014-5793 |
| DOI: |
10.1016/j.febslet.2008.05.051 |
| URL الوصول: |
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cc0f68c9bc4d22b90a3b5dd6e3c59a62 |
| Rights: |
OPEN |
| رقم الانضمام: |
edsair.doi.dedup.....cc0f68c9bc4d22b90a3b5dd6e3c59a62 |
| قاعدة البيانات: |
OpenAIRE |