Is Malassezia nana the main species in horses’ ear canal microbiome?
| العنوان: | Is Malassezia nana the main species in horses’ ear canal microbiome? |
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| المؤلفون: | Selene Dall' Acqua Coutinho, Ana Lúcia Aldrovandi, Lika Osugui |
| المصدر: | Brazilian Journal of Microbiology, Vol 47, Iss 3, Pp 770-774 Brazilian Journal of Microbiology, Volume: 47, Issue: 3, Pages: 770-774, Published: SEP 2016 Brazilian Journal of Microbiology Brazilian Journal of Microbiology v.47 n.3 2016 Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
| بيانات النشر: | Sociedade Brasileira de Microbiologia |
| مصطلحات موضوعية: | Male, 0301 basic medicine, 040301 veterinary sciences, Malassezia nana, Veterinary Microbiology, 030106 microbiology, lcsh:QR1-502, Polymerase Chain Reaction, Microbiology, lcsh:Microbiology, law.invention, 0403 veterinary science, 03 medical and health sciences, Ear canal, law, medicine, Animals, Horses, Polymerase chain reaction, Malassezia equina, Malassezia, biology, integumentary system, Microbiota, 04 agricultural and veterinary sciences, biology.organism_classification, Restriction enzyme, medicine.anatomical_structure, Genes, Bacterial, Malassezia slooffiae, Female, Cotton swab, Microbiome, Restriction fragment length polymorphism, Polymorphism, Restriction Fragment Length |
| الوصف: | The objective of this study was to characterize genotypically Malassezia spp. isolated from the external ear canal of healthy horses. Fifty-five horses, 39 (70.9%) males and 16 (29.1%) females, from different breeds and adults were studied. External ear canals were cleaned and a sterile cotton swab was introduced to collect cerumen. A total of 110 samples were cultured into Dixon medium and were incubated at 32 °C for up to 15 days. Macro- and micromorphology and phenotypic identification were performed. DNA was extracted, strains were submitted to polymerase chain reaction technique, and the products obtained were submitted to Restriction Fragment Length Polymorphism using the restriction enzymes BstCI and HhaI. Strains were sent off to genetic sequencing of the regions 26S rDNA D1/D2 and ITS1-5.8S-ITS2 rDNA. Malassezia spp. were isolated from 33/55 (60%) animals and 52/110 (47%) ear canals. No growth on Sabouraud dextrose agar was observed, confirming the lipid dependence of all strains. Polymerase chain reaction-Restriction fragment length polymorphism permitted the molecular identification of Malassezia nana - 42/52 (81%) and Malassezia slooffiae - 10/52 (19%). Sequencing confirmed RFLP identification. It was surprising that M. nana represented over 80% of the strains and no Malassezia equina was isolated in this study, differing from what was expected. |
| وصف الملف: | text/html |
| اللغة: | English |
| تدمد: | 1678-4405 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cbe13d2c89372c073576290808a6ec00 http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000300770&lng=en&tlng=en |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....cbe13d2c89372c073576290808a6ec00 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16784405 |
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