Purification and characterization of a peroxidase present in xilopodium exsudates of umbu plants (Spondias tuberosa A.)
| العنوان: | Purification and characterization of a peroxidase present in xilopodium exsudates of umbu plants (Spondias tuberosa A.) |
|---|---|
| المؤلفون: | M dos Santos Teixeira Pinto, J. M. Ribeiro, N. F. de Melo, Kvs Fernandes, F. P. de Araujo |
| المساهمون: | MARCIO DOS SANTOS TEIXEIRA PINTO, Universidade Federal do Tocantins - UFT, JULIANA MARTINS RIBEIRO, CPATSA, FRANCISCO PINHEIRO DE ARAUJO, CPATSA, NATONIEL FRANKLIN DE MELO, CPATSA, KÁTIA VALEVSKI SALES FERNANDES, Universidade Estadual do Norte Fluminense Darcy Ribeiro - UENF. |
| المصدر: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA-Alice) Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA African Journal of Biotechnology; Vol 14, No 21 (2015); 1838-1845 |
| بيانات النشر: | Academic Journals, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Polyacrylamide, Tssue brownig, Applied Microbiology and Biotechnology, Caatinga, Spondias tuberosa, chemistry.chemical_compound, Genetics, Root enzyme, Molecular Biology, Peroxidase, chemistry.chemical_classification, Guaiacol peroxidase, Chromatography, biology, biology.organism_classification, Tropolone, Umbu, Enzyme assay, Enzyme, Spondia tuberosa, chemistry, biology.protein, Sodium azide, Guaiacol, Agronomy and Crop Science, Root enzyme, tissue brownig, guaiacol peroxidase, oxidative metabolism, Biotechnology |
| الوصف: | Umbu plants are drought resistant trees which are able to store water and several other substances into its adapted root, named xylopodium. The exsudate from xilopodium is a natural solution rich in salts, sugars and a little concentration of proteins. In this work, we report the purification of a peroxidase (POX) from umbu xilopodium exsudate by direct extraction from polyacrylamide electrophoresis gels. Umbu POX showed optimum activity at pHs around 6.0 to 7.0 and high thermal resistance after incubation at 7 0°C for 6 min. POX activity present in crude extracts was more heat - resistant than in its purified form. When assayed with metal ions, umbu POX activity was shown to be inhibited by Mn 2+ and stimulated by Ca 2+ and Mg 2+ ; it was also inhibited by sodium azid e (concentrations higher then 1 mM) and not inhibited by either EDTA or tropolone. POX Km values for guaiacol and methylcatechol substrates were 6.83 and 22.25, respectively. The enzyme is proposed to be a guaiacol peroxidase and was seen to be located at higher concentrations in the outermost layers of xylopodium tissue, such as the endoderm. Made available in DSpace on 2017-05-03T23:47:05Z (GMT). No. of bitstreams: 1 Juliana2015.pdf: 464149 bytes, checksum: 2149a66d7fea93c9522d549e5ebb2137 (MD5) Previous issue date: 2015-07-08 |
| وصف الملف: | application/pdf |
| تدمد: | 1684-5315 |
| DOI: | 10.5897/ajb2015.14521 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cb6471e8c92ae020689e94ca186dc42b https://doi.org/10.5897/ajb2015.14521 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....cb6471e8c92ae020689e94ca186dc42b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16845315 |
|---|---|
| DOI: | 10.5897/ajb2015.14521 |