Identification of Novel microRNA Profiles Dysregulated in Plasma and Tissue of Abdominal Aortic Aneurysm Patients
| العنوان: | Identification of Novel microRNA Profiles Dysregulated in Plasma and Tissue of Abdominal Aortic Aneurysm Patients |
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| المؤلفون: | Laura Gálvez, Victoria Fornés-Ferrer, Joaquín Panadero, Pilar Medina, Manuel Miralles, Emma Plana, Silvia Navarro |
| المصدر: | International Journal of Molecular Sciences, Vol 21, Iss 4600, p 4600 (2020) INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES r-IIS La Fe. Repositorio Institucional de Producción Científica del Instituto de Investigación Sanitaria La Fe instname International Journal of Molecular Sciences Volume 21 Issue 13 |
| بيانات النشر: | MDPI AG, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Male, 0301 basic medicine, Pathology, medicine.medical_specialty, 030204 cardiovascular system & hematology, Article, Catalysis, Inorganic Chemistry, lcsh:Chemistry, 03 medical and health sciences, 0302 clinical medicine, abdominal aortic aneurysm, microRNA, Healthy volunteers, Aortic tissue, medicine, Humans, Physical and Theoretical Chemistry, Molecular Biology, Gene, lcsh:QH301-705.5, Spectroscopy, Aged, business.industry, Vascular disease, Gene Expression Profiling, Organic Chemistry, biomarkers, vascular disease, General Medicine, Middle Aged, Atherosclerosis, medicine.disease, Abdominal aortic aneurysm, Computer Science Applications, microRNAs, 030104 developmental biology, lcsh:Biology (General), lcsh:QD1-999, Case-Control Studies, miRNAs, Population study, Female, business, Thrombospondin-2, Aortic Aneurysm, Abdominal |
| الوصف: | microRNAs (miRNAs) are small RNAs that regulate different biological processes. Our objective was to identify miRNAs dysregulated in plasma and tissue of patients with abdominal aortic aneurysm (AAA) and explore new potential targets involved in AAA. Fifty-seven subjects were recruited for a plasma study (30 AAA patients, 16 healthy volunteers and 11 patients with atherosclerosis). The expression level of 179 miRNAs was screened in plasma from a subset of samples, and dysregulated miRNAs were validated in the entire study population. Dysregulated miRNAs were also quantified in aortic tissue of 21 AAA patients and 8 organ donors. Applying a gene set enrichment analysis, an interaction map of dysregulated miRNAs and their targets was built, and selected targets were quantified in tissue samples. miR-27b-3p and miR-221-3p were overexpressed in plasma of AAA patients compared with healthy controls, 1.6 times and 1.9 times, respectively. In AAA tissue, six miRNAs (miR-1, miR-27b-3p, miR-29b-3p, miR-133a-3p, miR-133b, and miR-195-5p) were underexpressed from 1.6 to 4.8 times and four miRNAs (miR-146a-5p, miR-21-5p, miR-144-3p, and miR-103a-3p) were overexpressed from 1.3 to 7.2 times. Thrombospondin-2, a target of miR-195-5p, was increased in AAA tissue and negatively correlated with the expression of miR-195-5p, suggesting their involvement in a common regulatory mechanism. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1661-6596 1422-0067 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ca7d3893c25081ef9d3c4fe66f1066bc https://www.mdpi.com/1422-0067/21/13/4600 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....ca7d3893c25081ef9d3c4fe66f1066bc |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16616596 14220067 |
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