Multivalency ensures persistence of a +TIP body at specialized microtubule ends
| العنوان: | Multivalency ensures persistence of a +TIP body at specialized microtubule ends |
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| المؤلفون: | Sandro M. Meier, Ana-Maria Farcas, Anil Kumar, Mahdiye Ijavi, Robert T. Bill, Jörg Stelling, Eric R. Dufresne, Michel O. Steinmetz, Yves Barral |
| المصدر: | Nature Cell Biology, 25 |
| بيانات النشر: | ETH Zurich, 2023. |
| سنة النشر: | 2023 |
| مصطلحات موضوعية: | Mitosis, Microtubules, Cell Biology |
| الوصف: | Microtubule plus-end tracking proteins (+TIPs) control microtubule specialization and are as such essential for cell division and morphogenesis. Here we investigated interactions and functions of the budding yeast Kar9 network consisting of the core +TIP proteins Kar9 (functional homologue of APC, MACF and SLAIN), Bim1 (orthologous to EB1) and Bik1 (orthologous to CLIP-170). A multivalent web of redundant interactions links the three +TIPs together to form a ‘+TIP body’ at the end of chosen microtubules. This body behaves as a liquid condensate that allows it to persist on both growing and shrinking microtubule ends, and to function as a mechanical coupling device between microtubules and actin cables. Our study identifes nanometre-scale condensates as efective cellular structures and underlines the power of dissecting the web of low-afnity interactions driving liquid–liquid phase separation in order to establish how condensation processes support cell function. Nature Cell Biology, 25 ISSN:1465-7392 ISSN:1476-4679 |
| وصف الملف: | application/application/pdf |
| اللغة: | English |
| تدمد: | 1465-7392 1476-4679 |
| DOI: | 10.3929/ethz-b-000590348 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c9dfb0e53a6eac2f665ea44a5cad9d21 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....c9dfb0e53a6eac2f665ea44a5cad9d21 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14657392 14764679 |
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| DOI: | 10.3929/ethz-b-000590348 |