Role of hepcidin in oxidative stress and cell death of cultured mouse renal collecting duct cells: protection against iron and sensitization to cadmium
| العنوان: | Role of hepcidin in oxidative stress and cell death of cultured mouse renal collecting duct cells: protection against iron and sensitization to cadmium |
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| المؤلفون: | Rachel P. L. van Swelm, Frank Thévenod, Natascha A. Wolff, Bettina Scharner, Stephanie Probst, Wing-Kee Lee, Eleni Roussa, Johannes Fels |
| المصدر: | Archives of Toxicology, 95, 2719-2735 Archives of Toxicology Archives of Toxicology, 95, 8, pp. 2719-2735 |
| بيانات النشر: | Springer Science and Business Media LLC, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | DFO, Male, 0301 basic medicine, Health, Toxicology and Mutagenesis, Apoptosis, Toxicology, medicine.disease_cause, CaV3.1, Mice, hemic and lymphatic diseases, DMT1, Cells, Cultured, chemistry.chemical_classification, Kidney, Cell Death, biology, General Medicine, medicine.anatomical_structure, Catalase, Female, Intracellular, Cadmium, inorganic chemicals, Programmed cell death, Iron, Deferoxamine, Binding, Competitive, Cell Line, 03 medical and health sciences, Hepcidins, Molecular Toxicology, Hepcidin, medicine, Animals, Gene Silencing, Kidney Tubules, Collecting, Reactive oxygen species, Binding Sites, 030102 biochemistry & molecular biology, fungi, nutritional and metabolic diseases, Molecular biology, Mice, Inbred C57BL, Oxidative Stress, Renal disorders Radboud Institute for Molecular Life Sciences [Radboudumc 11], 030104 developmental biology, chemistry, biology.protein, Metallothionein, Reactive Oxygen Species, Oxidative stress |
| الوصف: | The liver hormone hepcidin regulates systemic iron homeostasis. Hepcidin is also expressed by the kidney, but exclusively in distal nephron segments. Several studies suggest hepcidin protects against kidney damage involving Fe2+ overload. The nephrotoxic non-essential metal ion Cd2+ can displace Fe2+ from cellular biomolecules, causing oxidative stress and cell death. The role of hepcidin in Fe2+ and Cd2+ toxicity was assessed in mouse renal cortical [mCCD(cl.1)] and inner medullary [mIMCD3] collecting duct cell lines. Cells were exposed to equipotent Cd2+ (0.5–5 μmol/l) and/or Fe2+ (50–100 μmol/l) for 4–24 h. Hepcidin (Hamp1) was transiently silenced by RNAi or overexpressed by plasmid transfection. Hepcidin or catalase expression were evaluated by RT-PCR, qPCR, immunoblotting or immunofluorescence microscopy, and cell fate by MTT, apoptosis and necrosis assays. Reactive oxygen species (ROS) were detected using CellROX™ Green and catalase activity by fluorometry. Hepcidin upregulation protected against Fe2+-induced mIMCD3 cell death by increasing catalase activity and reducing ROS, but exacerbated Cd2+-induced catalase dysfunction, increasing ROS and cell death. Opposite effects were observed with Hamp1 siRNA. Similar to Hamp1 silencing, increased intracellular Fe2+ prevented Cd2+ damage, ROS formation and catalase disruption whereas chelation of intracellular Fe2+ with desferrioxamine augmented Cd2+ damage, corresponding to hepcidin upregulation. Comparable effects were observed in mCCD(cl.1) cells, indicating equivalent functions of renal hepcidin in different collecting duct segments. In conclusion, hepcidin likely binds Fe2+, but not Cd2+. Because Fe2+ and Cd2+ compete for functional binding sites in proteins, hepcidin affects their free metal ion pools and differentially impacts downstream processes and cell fate. |
| وصف الملف: | application/pdf |
| تدمد: | 1432-0738 0340-5761 |
| DOI: | 10.1007/s00204-021-03106-z |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c8dbdb654c10d20d84c7e04e916550e9 https://doi.org/10.1007/s00204-021-03106-z |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....c8dbdb654c10d20d84c7e04e916550e9 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14320738 03405761 |
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| DOI: | 10.1007/s00204-021-03106-z |