Detection ofCryptosporidium parvum oocysts using a microfluidic device equipped with the SUS micromesh and FITC-labeled antibody
| العنوان: | Detection ofCryptosporidium parvum oocysts using a microfluidic device equipped with the SUS micromesh and FITC-labeled antibody |
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| المؤلفون: | Masato Yoshino, Tadashi Matsunaga, Haruko Takeyama, Satoshi Haraguchi, Yoshio Ishimori, Atsushi Arakaki, Tomoyuki Taguchi, Kaneko Masao |
| المصدر: | Biotechnology and Bioengineering. 96:272-280 |
| بيانات النشر: | Wiley, 2006. |
| سنة النشر: | 2006 |
| مصطلحات موضوعية: | Cavity size, animal diseases, Microfluidics, Analytical chemistry, Antibodies, Protozoan, Fluorescent Antibody Technique, Bioengineering, Immunomagnetic separation, Applied Microbiology and Biotechnology, chemistry.chemical_compound, Tap water, Water Supply, parasitic diseases, Animals, Sodium dodecyl sulfate, Cryptosporidium parvum, Microchannel, Chromatography, biology, fungi, Oocysts, Water, Microfluidic Analytical Techniques, biology.organism_classification, chemistry, Porosity, Fluorescein-5-isothiocyanate, Biotechnology |
| الوصف: | Development of a microfluidic device equipped with micromesh for detection of Cryptosporidium parvum oocyst was reported. A micromesh consisting of 10 × 10 cavities was microfabricated on the stainless steel plate by laser ablation. Each cavity size, approximately 2.7 µm in diameter, was adopted to capture a single C. parvum oocyst. Under negative pressure operation, suspensions containing microbeads or C. parvum oocysts flowed into the microchannel. Due to strong non-specific adsorption of microbeads onto the PDMS microchannel surface during sample injection, the surface was treated with air plasma, followed by treatment with 1% sodium dodecyl sulfate (SDS) solution. This process reduced the non-specific adsorption of microbeads on the microchannel to 10% or less in comparison to a non-treated microchannel. This microfluidic device equipped with the SUS micromesh was further applied for the capture of C. parvum oocysts. Trapped C. parvum oocysts were visualized by staining with FITC-labeled anti-C. parvum oocyst antibody on a micromesh and counted under fluoroscopic observation. The result obtained by our method was consistent with that obtained by direct immunofluorescence assay coupled with immunomagnetic separation (DFA-IMS) method, indicating that the SUS micromesh is useful for counting of C. parvum oocysts. The newly designed microfluidic device exploits a geometry that allowed for the entrapment of oocysts on the micromesh while providing the rapid introduction of a series of reagents and washes through the microfluidic structure. Our data indicate that this microfluidic device is useful for high-throughput counting of C. parvum oocysts from tap water sample. Biotechnol. Bioeng. 2007;96: 272–280. © 2006 Wiley Periodicals, Inc. |
| تدمد: | 1097-0290 0006-3592 |
| DOI: | 10.1002/bit.21104 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c7f53a86fcb66f763ba5eaa5c63434cb https://doi.org/10.1002/bit.21104 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....c7f53a86fcb66f763ba5eaa5c63434cb |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10970290 00063592 |
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| DOI: | 10.1002/bit.21104 |