Sex-Dependent Sensory Phenotypes and Related Transcriptomic Expression Profiles Are Differentially Affected by Angelman Syndrome
| العنوان: | Sex-Dependent Sensory Phenotypes and Related Transcriptomic Expression Profiles Are Differentially Affected by Angelman Syndrome |
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| المؤلفون: | Lital Sharvit, Yonatan Feuermann, Lilach Simchi, Julia Panov, Prudhvi Raj Rayi, Hanoch Kaphzan, Lee Koyavski |
| المصدر: | Molecular Neurobiology. 56:5998-6016 |
| بيانات النشر: | Springer Science and Business Media LLC, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Male, 0301 basic medicine, Spatial Learning, Neuroscience (miscellaneous), Pain, Anxiety, Motor Activity, Biology, Hippocampus, Transcriptome, 03 medical and health sciences, Cellular and Molecular Neuroscience, 0302 clinical medicine, Memory, Angelman syndrome, medicine, UBE3A, Animals, Genetics, Sex Characteristics, Behavior, Animal, Temperature, Genetic disorder, Pain Perception, Fear, Sex hormone receptor, medicine.disease, Phenotype, Mice, Inbred C57BL, 030104 developmental biology, MRNA Sequencing, Neurology, Odorants, Exploratory Behavior, Autism, Female, Angelman Syndrome, 030217 neurology & neurosurgery |
| الوصف: | Angelman syndrome (AS) is a genetic disorder which entails autism, intellectual disability, lack of speech, motor deficits, and seizure susceptibility. It is caused by the lack of UBE3A protein expression, which is an E3-ubiquitin ligase. Despite AS equal prevalence in males and females, not much data on how sex affects the syndrome was reported. In the herein study, we thoroughly characterized many behavioral phenotypes of AS mice. The behavioral data acquired was analyzed with respect to sex. In addition, we generated a new mRNA sequencing dataset. We analyzed the coding transcriptome expression profiles with respect to the effects of genotype and sex observed in the behavioral phenotypes. We identified several neurobehavioral aspects, especially sensory perception, where AS mice either lack the male-to-female differences observed in wild-type littermates or even show opposed differences. However, motor phenotypes did not show male-to-female variation between wild-type (WT) and AS mice. In addition, by utilizing the mRNA sequencing, we identified genes and isoforms with expression profiles that mirror the sensory perception results. These genes are differentially regulated in the two sexes with inverse expression profiles in AS mice compared to WT littermates. Some of these are known pain-related and estrogen-dependent genes. The observed differences in sex-dependent neurobehavioral phenotypes and the differential transcriptome expression profiles in AS mice strengthen the evidence for molecular cross talk between Ube3a protein and sex hormone receptors or their elicited pathways. These interactions are essential for understanding Ube3a deletion effects, beyond its E3-ligase activity. |
| تدمد: | 1559-1182 0893-7648 |
| DOI: | 10.1007/s12035-019-1503-8 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c7aa9e6b462923aedf8dd7f267dad73e https://doi.org/10.1007/s12035-019-1503-8 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....c7aa9e6b462923aedf8dd7f267dad73e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15591182 08937648 |
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| DOI: | 10.1007/s12035-019-1503-8 |