Activation of Multiple Transcriptional Regulators by Growth Restriction in Pseudomonas aeruginosa
| العنوان: | Activation of Multiple Transcriptional Regulators by Growth Restriction in Pseudomonas aeruginosa |
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| المؤلفون: | Doo Hwan Yeom, Su-Jin Im, Soo-Kyoung Kim, Joon-Hee Lee |
| المصدر: | Molecules and Cells |
| بيانات النشر: | Korean Society for Molecular and Cellular Biology, 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | antibiotic resistance, AntR, Regulator, Repressor, medicine.disease_cause, Article, growth restriction, Microbiology, Bacterial Proteins, Acetyltransferases, medicine, Receptor, Secondary metabolism, Molecular Biology, QscR, biology, Pseudomonas aeruginosa, SoxR, Quorum Sensing, Pathogenic bacteria, Gene Expression Regulation, Bacterial, Cell Biology, General Medicine, biology.organism_classification, Anti-Bacterial Agents, Repressor Proteins, Oxidative Stress, Quorum sensing, Bacteria, Disinfectants |
| الوصف: | Growth restriction by antibiotics is a common feature that pathogenic bacteria must overcome for survival. The struggle of bacteria to escape from growth restriction eventually results in development of antibiotic-resistance through the expression of a set of genes. Here we found that some physiologically important transcriptional regulators of Pseudomonas aeruginosa including QscR, a quorum sensing (QS) receptor, SoxR, a superoxide sensor-regulator, and AntR, a regulator of anthranilate-related secondary metabolism, are activated by various growth-restricted conditions. We generated the growth-restricted conditions by various methods, such as overexpression of PA2537 and treatment with antibiotics or disinfectants. The overexpression of PA2537, encoding an acyltransferase homologue, tightly restricted the growth of P. aeruginosa and significantly activated QscR during the growth restriction. Similarly, treatments with gentamycin, tetracycline, and ethanol also activated QscR near their minimal inhibitory concentrations (MICs). Some non-QS regulators, such as AntR and SoxR, were also activated near the MICs in the same conditions. However, LasR and PqsR, other QS receptors of P. aeruginosa, were not activated, suggesting that only a specific set of transcriptional regulators is activated by growth restriction. Since paraquat, a super-oxide generator, significantly activated QscR and AntR, we suggest that the oxidative stress generated by growth restriction may be partly involved in this phenomenon. |
| تدمد: | 0219-1032 1016-8478 |
| DOI: | 10.14348/molcells.2014.0105 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c7000f8a47bb34ae71fc18aca1499b07 https://doi.org/10.14348/molcells.2014.0105 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....c7000f8a47bb34ae71fc18aca1499b07 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 02191032 10168478 |
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| DOI: | 10.14348/molcells.2014.0105 |