Nerve growth factor (NGF) receptors in a central nervous system glial cell line: Upregulation by NGF and brain-derived neurotrophic factor
| العنوان: | Nerve growth factor (NGF) receptors in a central nervous system glial cell line: Upregulation by NGF and brain-derived neurotrophic factor |
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| المؤلفون: | Alberta Leon, Stephen D. Skaper, S Romanello, Lucia Petrelli, P. E. Spoerri, Alessandro Negro, R. Dal Toso |
| المصدر: | Journal of Neuroscience Research. 33:82-90 |
| بيانات النشر: | Wiley, 1992. |
| سنة النشر: | 1992 |
| مصطلحات موضوعية: | Nerve Tissue Proteins, Receptors, Nerve Growth Factor, Biology, Cell Line, Iodine Radioisotopes, Cellular and Molecular Neuroscience, Downregulation and upregulation, Neurotrophic factors, Tumor Cells, Cultured, Animals, Nerve Growth Factors, Microscopy, Immunoelectron, Brain-derived neurotrophic factor, Brain Neoplasms, Brain-Derived Neurotrophic Factor, Endoplasmic reticulum, Glioma, Immunogold labelling, Blotting, Northern, Rats, Up-Regulation, Cell biology, Microscopy, Electron, Nerve growth factor, nervous system, Cell culture, Immunology, biology.protein, Neuroglia, Neurotrophin |
| الوصف: | The neurotrophic proteins nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) are related in their primary amino acid structures. In this study we investigated the extent to which the low-affinity NGF receptor (LNGFR) in C6 glioma cells can discriminate between the neurotrophins NGF and BDNF. LNGFR-immunoreactivity (IR) was studied in C6 cells treated for 16 hr with NGF (50 ng/ml) or BDNF (10 ng/ml), using immunogold labelling and electron microscopic morphometric analysis. The cells were exposed to the anti-NGFR antibody 192-IgG, followed by immunoglobulin conjugated with colloidal gold. Untreated C6 cells exhibited some surface gold label (positive LNGFR-IR). Cells treated with NGF or BDNF displayed significantly increased LNGFR-IR on all surfaces in terms of gold labeling, which was more pronounced in NGF-treated cells. LNGFR-IR was also localized in coated endocytotic vesicles, in smooth endoplasmic reticulum, and in secondary multivesicular lysosomes in neurotrophin-treated and untreated cells. The increase in LNGFR protein was further substantiated by a correspondingly higher content of LNGFR mRNA detected after 15 hr of either NGF or BDNF treatment. These results suggest that the LNGFR in glial cells can be upregulated by the structurally related neurotrophins NGF and BDNF. |
| تدمد: | 1097-4547 0360-4012 |
| DOI: | 10.1002/jnr.490330111 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c6fecc8e8c989a0937353c502f3f9513 https://doi.org/10.1002/jnr.490330111 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....c6fecc8e8c989a0937353c502f3f9513 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10974547 03604012 |
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| DOI: | 10.1002/jnr.490330111 |