التفاصيل البيبلوغرافية
| العنوان: |
A new approach to design artificial 3D micro-niches with combined chemical, topographical and rheological cues |
| المؤلفون: |
Zhang Yue, Wilhelm W. Chen, Richard De Mets, Vincent Studer, Celine Stoecklin, Virgile Viasnoff, Eileen Fong |
| المساهمون: |
School of Materials Science and Engineering |
| بيانات النشر: |
Cold Spring Harbor Laboratory, 2018. |
| سنة النشر: |
2018 |
| مصطلحات موضوعية: |
0301 basic medicine, Biomimetic materials, Materials [Engineering], Lineage commitment, Computer science, Biomedical Engineering, 02 engineering and technology, 3D Cell Culture, 021001 nanoscience & nanotechnology, General Biochemistry, Genetics and Molecular Biology, Biomaterials, Extracellular matrix, 03 medical and health sciences, 3D cell culture, 030104 developmental biology, Biomimetic Materials, Nanotopography, 0210 nano-technology, Biological system, Sensory cue |
| الوصف: |
The in vitro methods to recapitulate environmental cues around cells are usually optimized to test a specific property of the environment (biochemical nature or the stiffness of the extra cellular matrix (ECM), or nanotopography) for its capability to induce defined cell behaviors (lineage commitment, migration). Approaches that combine different environmental cues in 3D to assess the biological response of cells to the spatial organization of different biophysical and biochemical cues are growingly being developed. We demonstrate how the lamination of through-hole polymeric bio-functionalized membranes can be implemented to create complex bona fide micro-niches with differential 3D environmental properties using photoactive materials. Our approach enables to create micro-niches ranging in size from single cells to cell aggregates. They are bio-functionalized in 3D simultaneously with topographical featured, protein patterns and structured ECM surrogate with 1 micrometer resolution. We demonstrate how these niches extend in 3D the ability to pattern cells. We exemplify how they can be used to standardize cells shapes in 3D and to trigger the apico-basal polarization of single epithelial cells. |
| اللغة: |
English |
| DOI: |
10.1101/291104 |
| URL الوصول: |
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c660537b832141ada1ad3ef6aa05d785 |
| Rights: |
OPEN |
| رقم الانضمام: |
edsair.doi.dedup.....c660537b832141ada1ad3ef6aa05d785 |
| قاعدة البيانات: |
OpenAIRE |