2-Deoxyglucose and Newcastle Disease Virus Synergize to Kill Breast Cancer Cells by Inhibition of Glycolysis Pathway Through Glyceraldehyde3-Phosphate Downregulation
| العنوان: | 2-Deoxyglucose and Newcastle Disease Virus Synergize to Kill Breast Cancer Cells by Inhibition of Glycolysis Pathway Through Glyceraldehyde3-Phosphate Downregulation |
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| المؤلفون: | Ahmed Majeed Al-Shammari, Nahi Y. Yaseen, Amer Hasan Abdullah, Zainab Majid Allami |
| المصدر: | Frontiers in Molecular Biosciences Frontiers in Molecular Biosciences, Vol 6 (2019) |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, breast cancer model, cancer metabolism, Biochemistry, Genetics and Molecular Biology (miscellaneous), Biochemistry, 03 medical and health sciences, Glycolysis Inhibition, 0302 clinical medicine, Downregulation and upregulation, glycolysis inhibition, Molecular Biosciences, Molecular Biology, lcsh:QH301-705.5, Glyceraldehyde 3-phosphate dehydrogenase, Original Research, biology, Chemistry, Glucose analog, Warburg effect, 030104 developmental biology, lcsh:Biology (General), Apoptosis, Anaerobic glycolysis, 030220 oncology & carcinogenesis, Cancer cell, biology.protein, Cancer research, virotherapy |
| الوصف: | Targeting cancer cells metabolism is promising strategy in inhibiting cancer cells progression that are known to exhibit increased aerobic glycolysis. We used the glucose analog 2-Deoxyglucose (2-DG) as a competitor molecule of glucose. To further enhance the effectiveness of 2-DG, the Newcastle disease virus (NDV) was used as a combination virotherapy to enhance the anti-tumor effect. Human and mouse-breast cancer cells were treated by NDV and/or 2-DG. The effect was analyzed by study cell viability, apoptosis and level of glyceraldehyde3-phosphate (GAPDH) by ELISA and QPCR assays. Synergistic cytotoxicity was found after a 72-h treatment of human- and mouse-breast cancer cells with 2-DG in combination with NDV at different concentrations. The synergistic cytotoxicity was accompanied by apoptotic cell death and GAPDH downregulation and inhibition to glycolysis product pyruvate. The combination treatment showed significant tumor growth inhibition compared to single treatments in vivo. Our results suggest the effectiveness of a novel strategy for anti-breast cancer therapy through glycolysis inhibition and GAPDH downregulation. |
| تدمد: | 2296-889X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c5c9dfffb280d2a30ecf2b9fda39d5fb https://pubmed.ncbi.nlm.nih.gov/31612140 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....c5c9dfffb280d2a30ecf2b9fda39d5fb |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 2296889X |
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