Effects of ipriflavone-loaded mesoporous nanospheres on the differentiation of endothelial cells and their modulation by macrophages
العنوان: | Effects of ipriflavone-loaded mesoporous nanospheres on the differentiation of endothelial cells and their modulation by macrophages |
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المؤلفون: | María José Feito, Daniel Arcos, María Concepción Serrano, María Vallet-Regí, Laura Casarrubios, María Teresa Portolés, Alberto Polo-Montalvo |
المصدر: | Nanomaterials Volume 11 Issue 5 E-Prints Complutense. Archivo Institucional de la UCM instname Nanomaterials, Vol 11, Iss 1102, p 1102 (2021) E-Prints Complutense: Archivo Institucional de la UCM Universidad Complutense de Madrid |
بيانات النشر: | MDPI, 2021. |
سنة النشر: | 2021 |
مصطلحات موضوعية: | 0301 basic medicine, mesoporous nanospheres, Angiogenesis, General Chemical Engineering, Phagocytosis, 02 engineering and technology, ipriflavone, Endocytosis, Article, 03 medical and health sciences, vascular endothelial growth factor receptor 2, Macrophage, endocytosis, General Materials Science, Progenitor cell, QD1-999, endothelial progenitor cells, Materiales, Chemistry, Kinase insert domain receptor, 021001 nanoscience & nanotechnology, M2 Macrophage, In vitro, Cell biology, macrophages, 030104 developmental biology, embryonic structures, cardiovascular system, 0210 nano-technology, circulatory and respiratory physiology |
الوصف: | Angiogenic biomaterials for bone repair are being designed to promote vascularization and optimize tissue regeneration. The use of nanoparticles of bioactive materials loaded with different drugs represents an interesting strategy to stimulate osteogenesis and angiogenesis and to inhibit bone resorption. Ipriflavone (IP) prevents osteoporosis by inhibiting osteoclast activity and promoting preosteoblast differentiation into mature osteoblasts. Since endothelial progenitor cells (EPCs) are involved in the formation of blood vessels which are necessary for tissue regeneration, the isolation and characterization of porcine EPCs have been carried out in this work to evaluate the in vitro effects of unloaded (NanoMBGs) and IP-loaded nanospheres (NanoMBG-IPs) designed to stimulate osteogenesis. Because different signals between vascular and nonvascular cells are also essential to initiate angiogenic events, the potential modulating role of macrophages has been also evaluated by studying the expression of vascular endothelial growth factor receptor 2 (VEFGR2) as a specific marker for EPC differentiation under different culture conditions: a) EPCs in monoculture treated with NanoMBGs or NanoMBG-IPs, b) EPCs treated with conditioned media from basal, proinflammatory M1 and reparative M2 macrophages previously treated with NanoMBGs or NanoMBG-IPs, c) EPCs cocultured with macrophages in the presence of NanoMBGs or NanoMBG-IPs, and d) EPCs cocultured with M2d angiogenic macrophages. Moreover, the endocytic mechanisms by which these nanospheres are incorporated by EPCs have been identified by using six endocytosis inhibitors (i.e. wortmannin, genistein, cytochalasin B, cytochalasin D, phenylarsine oxide and chlorpromazine) and before the addition of NanoMBGs labeled with fluorescein isothiocyanate. The results evidence the great potential of both NanoMBGs and NanoMBG-IPs to enhance VEFGR2 expression, directly related to angiogenesis, after intracellular incorporation by EPCs through different endocytic mechanisms including clathrin-dependent endocytosis, as the main entry mechanism, but also phagocytosis and caveolae-mediated uptake. The treatment of EPCs with culture media from basal, M1 and M2 macrophages and the development of cocultures of EPCs with macrophages in the absence and presence of these nanomaterials have also confirmed the maintenance of their angiogenic effect on EPCs even in the presence of phagocytic cells. |
وصف الملف: | application/pdf |
اللغة: | English |
URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c5827988dda00671d155794dbae9e83f https://eprints.ucm.es/id/eprint/65084/ |
Rights: | OPEN |
رقم الانضمام: | edsair.doi.dedup.....c5827988dda00671d155794dbae9e83f |
قاعدة البيانات: | OpenAIRE |
الوصف غير متاح. |