Expression analysis of argonaute, Dicer-like, and RNA-dependent RNA polymerase genes in cucumber (Cucumis sativus L.) in response to abiotic stress
| العنوان: | Expression analysis of argonaute, Dicer-like, and RNA-dependent RNA polymerase genes in cucumber (Cucumis sativus L.) in response to abiotic stress |
|---|---|
| المؤلفون: | Dandi Liang, Ling Zhang, Defang Gan, Qinghui Lu, Qiqi Zhang, Feng Yang, Mengdan Zhan, Keling Hu, Wenjuan Xu |
| المصدر: | Journal of Genetics. 96:235-249 |
| بيانات النشر: | Springer Science and Business Media LLC, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | Ribonuclease III, 0301 basic medicine, Genetics, Abiotic stress, RNA-dependent RNA polymerase, Biology, Argonaute, RNA-Dependent RNA Polymerase, 03 medical and health sciences, 030104 developmental biology, Gene Expression Regulation, Plant, Stress, Physiological, RNA interference, Multigene Family, Argonaute Proteins, Gene expression, biology.protein, Gene family, Amino Acid Sequence, Cucumis sativus, Gene, Phylogeny, Plant Proteins, Dicer |
| الوصف: | Posttranscriptional control of gene expression can be achieved through RNA interference when the activities of Dicer-like (DCL), argonaute (AGO) and RNA-dependent RNA polymerase (RDR) proteins are significant. In this study, we analysed the expression of seven AGO, five DCL and eight RDR genes in cucumber under cold, heat, hormone, salinity and dehydration treatments using quantitative reverse-transcription PCR (qRT-PCR). All CsAGO, CsDCL and CsRDR genes were differentially expressed under abiotic stress treatment. In response to abiotic stress treatment, most genes were expressed at higher levels in flowers or stems than in other organs, whereas some CsAGOs (CsAGO1c, CsAGO6 and CsAGO7) and CsRDRs (CsRDR1d and CsRDR2) were highly expressed in roots during dehydration treatment. The expression patterns indicate that most CsDCLs, CsAGOs and CsRDRs respond to abiotic stress, and stems or flowers are the most sensitive organs, followed by roots. This is the first report of expression analysis of all CsDCL, CsAGO and CsRDR family genes in cucumber under abiotic stress, which provides basic information and insights into the putative roles of these genes in abiotic stress. The results of this study should serve as a basis for further functional characterization of these gene families in cucumber and related Cucurbitaceae species. |
| تدمد: | 0973-7731 0022-1333 |
| DOI: | 10.1007/s12041-017-0758-y |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c568f09ad867e335c66d0f4df75970f0 https://doi.org/10.1007/s12041-017-0758-y |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....c568f09ad867e335c66d0f4df75970f0 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 09737731 00221333 |
|---|---|
| DOI: | 10.1007/s12041-017-0758-y |