Flavobacterium bizetiae sp. nov., isolated from diseased freshwater fish in Canada at the end of the 1970s
| العنوان: | Flavobacterium bizetiae sp. nov., isolated from diseased freshwater fish in Canada at the end of the 1970s |
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| المؤلفون: | Priscilla Leclerc, Chloé Abry, Alexis Criscuolo, Estelle Mühle, Hans-Jürgen Busse, Olivier Chesneau, Dominique Clermont, Gogoa-Marthe Goly, Peter Kämpfer, Jean-François Bernardet |
| المساهمون: | Collection de l'Institut Pasteur (CIP), Institut Pasteur [Paris], Hub Bioinformatique et Biostatistique - Bioinformatics and Biostatistics HUB, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Veterinärmedizinische Universität Wien, Justus-Liebig-Universität Gießen (JLU), Virologie et Immunologie Moléculaires (VIM (UR 0892)), Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), We are grateful to our colleagues of the Spanish Type Culture Collection for their gift of strains CECT 7796T, type strain of F. collinsii, and CECT 7844T, type strain of F. plurextorum, that enabled us to perform this study. Please note that both strains cannot be distributed by CIP according to the agreed Material Transfer Agreement. The genome of CECT 7796T was sequenced at Institut Pasteur by the Plateforme de Microbiologie Mutualisée (P2M) for the taxonomic purpose of this study and its accession number is CADCST01., Institut Pasteur [Paris] (IP), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Justus-Liebig-Universität Gießen = Justus Liebig University (JLU) |
| المصدر: | International Journal of Systematic and Evolutionary Microbiology International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, 2020, ⟨10.1099/ijsem.0.004576⟩ International Journal of Systematic and Evolutionary Microbiology, 2021, 71 (1), pp.004576. ⟨10.1099/ijsem.0.004576⟩ |
| بيانات النشر: | HAL CCSD, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | food.ingredient, Gliding motility, Biology, Microbiology, Flavobacterium, 03 medical and health sciences, chemistry.chemical_compound, food, Agar, genome phylogeny, 14. Life underwater, Melibiose, freshwater, Ecology, Evolution, Behavior and Systematics, 030304 developmental biology, fish, 0303 health sciences, Strain (chemistry), Phylogenetic tree, 030306 microbiology, General Medicine, 16S ribosomal RNA, biology.organism_classification, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, chemistry, Bacteria |
| الوصف: | Genome sequence analysis of two strains collected in Canada at the end of the 1970s and deposited in 1998 at the Collection de l’Institut Pasteur has led to the taxonomic description of a novel fish-associated species in the genus Flavobacterium . Both strains, CIP 105534T and CIP 105535, were yellow-pigmented, Gram-stain-negative, non-spore-forming rod-shaped bacteria that exhibited gliding motility. They grew aerobically in a temperature range from 5 to 30 °C with optimal growth at 25 °C on trypticase soy or Reasoner's 2A agar but they did not grow on marine agar. Their major fatty acid profiles were similar, consisting of iso-C15 : 0, C16 : 1 ω7c and/or iso-C15 : 0 2-OH (shown as summed feature 3), C16 : 0 3-OH, iso-C17 : 0 3-OH and C16 : 0. The major polyamine was sym-homospermidine. Phosphatidylethanolamine and, most notably, ornithine-containing lipid OL2 and unidentified aminophospholipid APL1 were major polar lipids. A yellow pigment spot was visible after chromatographic analysis. The predominant respiratory quinone was MK-6. The G+C content of the two genomes was 34 mol% and their size was around 5.8 Mb. Comparison of the 16S rRNA gene sequences with those of the closely related type strains showed high levels of relatedness with Flavobacterium collinsii and Flavobacterium pectinovorum . All average nucleotide identity (ANI) and digital DNA–DNA hybridization values estimated against publicly available Flavobacterium genome assemblies were lower than 90 and 30 %, respectively. Phylogenetic, phenotypic and chemotaxonomic data indicated that the two strains represent a novel species of the genus Flavobacterium , for which the name Flavobacterium bizetiae sp. nov. is proposed. The type strain is CIP 105534T (=LMG 1342T). The unique ability of F. bizetiae to use melibiose as a sole source of carbon could provide a simple phenotypic test to discriminate F. bizetiae from its closest relatives. |
| اللغة: | English |
| تدمد: | 1466-5026 1466-5034 |
| DOI: | 10.1099/ijsem.0.004576⟩ |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c558fcff60640c0c4647683c4e7e5396 https://hal-pasteur.archives-ouvertes.fr/pasteur-03082360 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....c558fcff60640c0c4647683c4e7e5396 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14665026 14665034 |
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| DOI: | 10.1099/ijsem.0.004576⟩ |