Methylated Host Cell Gene Promoters and Human Papillomavirus Type 16 and 18 Predicting Cervical Lesions and Cancer
| العنوان: | Methylated Host Cell Gene Promoters and Human Papillomavirus Type 16 and 18 Predicting Cervical Lesions and Cancer |
|---|---|
| المؤلفون: | Ivan Sabol, Ivan Fistonić, Pavao Planinić, Ante Ćorušić, Magdalena Grce, Goran Grubišić, Nina Milutin Gašperov |
| المصدر: | PLoS ONE, Vol 10, Iss 6, p e0129452 (2015) PLoS ONE PLoS One |
| بيانات النشر: | Public Library of Science (PLoS), 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Adult, Gene Expression Regulation, Viral, Bisulfite sequencing, Uterine Cervical Neoplasms, lcsh:Medicine, Cervix Uteri, Biology, Cell Line, Cytosine, Young Adult, medicine, Humans, Promoter Regions, Genetic, lcsh:Science, Cervical cancer, Regulation of gene expression, Human papillomavirus 16, Multidisciplinary, Human papillomavirus 18, Papillomavirus Infections, lcsh:R, Cancer, Basic Medical Sciences, Promoter, Methylation, DNA Methylation, Middle Aged, medicine.disease, Molecular biology, methylation, HPV, cancer, 3. Good health, CpG site, DNA methylation, DNA, Viral, CpG Islands, Female, lcsh:Q, Biomarkers, Research Article |
| الوصف: | Change in the host and/or human papillomavirus (HPV) DNA methylation profile is probably one of the main factors responsible for the malignant progression of cervical lesions to cancer. To investigate those changes we studied 173 cervical samples with different grades of cervical lesion, from normal to cervical cancer. The methylation status of nine cellular gene promoters, CCNA1, CDH1, C13ORF18, DAPK1, HIC1, RARβ2, hTERT1, hTERT2 and TWIST1 was investigated by Methylation Specific Polymerase Chain Reaction (MSP). The methylation of HPV18 L1-gene was also investigated by MSP, while the methylated cytosines within four regions, L1, 5’LCR, enhancer, and promoter of the HPV16 genome covering 19 CpG sites were evaluated by bisulfite sequencing. Statistically significant methylation biomarkers distinguishing between cervical precursor lesions from normal cervix were primarily C13ORF18 and secondly CCNA1, and those distinguishing between cervical cancer from normal or cervical precursor lesions were CCNA1, C13ORF18, hTERT1, hTERT2 and TWIST1. In addition, the methylation analysis of individual CpG sites of the HPV16 genome in different sample groups, notably 7455 and 7694 sites, proved to be more important than the overall methylation frequency. The majority of HPV18 positive samples contained both methylated and unmethylated L1 gene, and samples with L1-gene methylated forms alone had better prognosis when correlated with the host cell gene promoters’ methylation profiles. In conclusion, both cellular and viral methylation biomarkers should be used for monitoring cervical lesion progression to prevent invasive cervical cancer. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1932-6203 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c4eb0c9ac20fe1c6c310e1871ee6e3b7 http://europepmc.org/articles/PMC4461273?pdf=render |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....c4eb0c9ac20fe1c6c310e1871ee6e3b7 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
|---|