Increase in Lectin Binding Sites on Epithelial Cells by Chronic Bladder Infection in Rats
| العنوان: | Increase in Lectin Binding Sites on Epithelial Cells by Chronic Bladder Infection in Rats |
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| المؤلفون: | Kenji Watanabe, Tatsuo Nakagawa, Yoshihiro Kitami, Akimi Ogawa, Nobuteru Usuda, Toru Hanai |
| المصدر: | Urologia Internationalis. 56:90-95 |
| بيانات النشر: | S. Karger AG, 1996. |
| سنة النشر: | 1996 |
| مصطلحات موضوعية: | Male, Glycoconjugate, Urology, Receptors, Cell Surface, urologic and male genital diseases, Bacterial Adhesion, Epithelium, Lectins, Cystitis, medicine, Animals, Rats, Wistar, Receptor, chemistry.chemical_classification, Urinary bladder, biology, Lectin, biology.organism_classification, Molecular biology, Rats, Staining, Microscopy, Electron, medicine.anatomical_structure, chemistry, Canavalia ensiformis, Biotinylation, Chronic Disease, Immunology, biology.protein, Immunohistochemistry, Female |
| الوصف: | Using lectin histochemistry we assessed whether chronic bladder infection modifies carbohydrate residues of glycoconjugates on uroepithelial cells in rats. The bladder infection was produced by implanting a knotted silk thread with Escherichia coli into the bladder. One or 4 weeks after the implantation the bladder was excised, incubated with sixteen biotinylated lectins and stained. The bladder epithelia as a whole stained more strongly positive for eight lectins in the infected rats than in the control rats having a sterile silk thread in the bladder. In the infected rats, the superficial epithelial layer that stained negative for Arachis hypogaea (PNA) in the controls became strongly positive for PNA, whereas the middle and deep epithelial layers increased in staining for Canavalia ensiformis and six other lectins. These results indicate that chronic bladder infection increases carbohydrate residues on uroepithelial cells and may facilitate bacterial adherence to uroepithelial cells. |
| تدمد: | 1423-0399 0042-1138 |
| DOI: | 10.1159/000282818 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c424a610ce9b59c30debb56c30aa9bfd https://doi.org/10.1159/000282818 |
| رقم الانضمام: | edsair.doi.dedup.....c424a610ce9b59c30debb56c30aa9bfd |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14230399 00421138 |
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| DOI: | 10.1159/000282818 |