Gastric smooth muscle cells manifest an abnormal phenotype in Parkinson’s disease rats with gastric dysmotility
| العنوان: | Gastric smooth muscle cells manifest an abnormal phenotype in Parkinson’s disease rats with gastric dysmotility |
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| المؤلفون: | Yan-Yan Fan, Li-Fei Zheng, Xiao-Yu Liu, Xiao-Lin Xiu, Jin-Xia Zhu |
| المصدر: | Cell and Tissue Research. 381:217-227 |
| بيانات النشر: | Springer Science and Business Media LLC, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Male, 0301 basic medicine, medicine.medical_specialty, Gastroparesis, Histology, Carbachol, Myosin light-chain kinase, Parkinson's disease, Myocytes, Smooth Muscle, Substantia nigra, Pathology and Forensic Medicine, Rats, Sprague-Dawley, 03 medical and health sciences, 0302 clinical medicine, Internal medicine, Myosin, Serum response factor, medicine, Animals, Chemistry, Stomach, Smooth muscle layer, Parkinson Disease, Cell Biology, medicine.disease, Rats, 030104 developmental biology, Endocrinology, Gastrointestinal Motility, 030217 neurology & neurosurgery, Muscle Contraction, medicine.drug |
| الوصف: | Gastroparesis is a common symptom in Parkinson's disease (PD) and whether any change occurs in gastric smooth muscle cells (SMCs) of PD patients is unclear. We previously reported that rats with bilateral substantia nigra lesions induced by 6-hydroxydopamine (6-OHDA), referred to as 6-OHDA rats, manifest typical gastroparesis. In the present study, we further investigate the underlying mechanism. By means of an organ bath system and an implantable radiotelemetry system, both a weakened contractile force of gastric circular smooth muscle and gastric myoelectric activity were detected in the 6-OHDA rats and phasic and tonic contractions elicited by carbachol or high concentration of potassium were significantly reduced in gastric circular muscle strips. A thickened smooth muscle layer was observed under a light microscope and an ultrastructure of hypertrophic SMCs, with increased caveolae and decreased dense bodies, was observed under transmission electron microscope. Furthermore, the mRNA and protein expression levels of contractile markers (myosin light chain 20, myosin heavy chain 11 and α-smooth muscle actin) and the transcription factor serum response factor (SRF) were significantly decreased, while the TNFα and IL-1β content was increased in the 6-OHDA rats. These results suggest that the decreased contractile force in 6-OHDA rats may be associated with the phenotypic abnormality observed in SMCs, which is due to downregulated contractile proteins induced by decreased SRF expression in the inflammatory muscular microenvironment. |
| تدمد: | 1432-0878 0302-766X |
| DOI: | 10.1007/s00441-020-03214-9 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c35d730b3366dcbe9c6c6bd6e5c6746b https://doi.org/10.1007/s00441-020-03214-9 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....c35d730b3366dcbe9c6c6bd6e5c6746b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14320878 0302766X |
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| DOI: | 10.1007/s00441-020-03214-9 |