Insights into early ontogenesis: characterization of stress and development key genes of pikeperch (Sander lucioperca) in vivo and in vitro
| العنوان: | Insights into early ontogenesis: characterization of stress and development key genes of pikeperch (Sander lucioperca) in vivo and in vitro |
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| المؤلفون: | Bianka Grunow, Alexander Rebl, Marieke Verleih, George P. Franz, Nadine Schäfer, Ronald M. Brunner, Henrike Rebl, Yagmur Kaya, Marcus Stüeken, Julien Alban Nguinkal, Tom Goldammer |
| المصدر: | Fish Physiology and Biochemistry |
| بيانات النشر: | Springer Netherlands, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Fish Proteins, Embryo, Nonmammalian, Physiology, Ontogeny, Cell Culture Techniques, Embryonic Development, Aquaculture, Aquatic Science, Biology, Bone morphogenetic protein, Biochemistry, Article, 03 medical and health sciences, 0302 clinical medicine, Transcription (biology), Animal welfare, Stress, Physiological, medicine, Animals, Pikeperch, Yolk sac, Fish cell line, Receptor, Gene, Cells, Cultured, 030304 developmental biology, 0303 health sciences, Stress response, Gene Expression Regulation, Developmental, Embryo, General Medicine, Cell biology, Perciformes, Bone morphogenetic protein 7, medicine.anatomical_structure, Early ontogenesis, Transcriptome, 030217 neurology & neurosurgery |
| الوصف: | There are still numerous difficulties in the successful farming of pikeperch in the anthropogenic environment of various aquaculture systems, especially during early developmental steps in the hatchery. To investigate the physiological processes involved on the molecular level, we determined the basal expression patterns of 21 genes involved in stress and immune responses and early ontogenesis of pikeperch between 0 and 175 days post hatch (dph). Their transcription patterns most likely reflect the challenges of growth and feed conversion. The gene coding for apolipoprotein A (APOE) was strongly expressed at 0 dph, indicating its importance for yolk sac utilization. Genes encoding bone morphogenetic proteins 4 and 7 (BMP4,BMP7), creatine kinase M (CKM), and SRY-box transcription factor 9 (SOX9) were highly abundant during the peak phases of morphological changes and acclimatization processes at 4–18 dph. The high expression of genes coding for peroxisome proliferator-activated receptors alpha and delta (PPARA,PPARD) at 121 and 175 dph, respectively, suggests their importance during this strong growth phase of juvenile stages. As an alternative experimental model to replace further in vivo investigations of ontogenetically important processes, we initiated the first approach towards a long-lasting primary cell culture from whole pikeperch embryos. The present study provides a set of possible biomarkers to support the monitoring of pikeperch farming and provides a first basis for the establishment of a suitable cell model of this emerging aquaculture species. |
| اللغة: | English |
| تدمد: | 1573-5168 0920-1742 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c2418ad6b60be01ceb90ee3bd67ae69c http://europepmc.org/articles/PMC8026417 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....c2418ad6b60be01ceb90ee3bd67ae69c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15735168 09201742 |
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