Identification of tumour-reactive lymphatic endothelial cells capable of inducing progression of gastric cancer
| العنوان: | Identification of tumour-reactive lymphatic endothelial cells capable of inducing progression of gastric cancer |
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| المؤلفون: | Mao Tokumoto, Masakazu Yashiro, Hiroaki Tanaka, Naoshi Kubo, Yukie Tauchi, Hiroaki Kasashima, Kento Kurata, Masaichi Ohira, Katsunobu Sakurai, Kiyoshi Maeda, Kazuya Muguruma, Ryosuke Amano, Kosei Hirakawa, Takahiro Toyokawa, Kenjiro Kimura |
| المصدر: | British Journal of Cancer |
| بيانات النشر: | Springer Science and Business Media LLC, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Cancer Research, Stromal cell, medicine.medical_treatment, Physiology, Biology, Metastasis, lymphatic endothelial cells, Cell Movement, Stomach Neoplasms, lymph nodes, Tumor Microenvironment, medicine, Humans, metastasis, Molecular Diagnostics, Lymph node, Cells, Cultured, Cell Proliferation, Inflammation, Tumor microenvironment, Gene Expression Profiling, gastric cancer, Growth factor, fungi, Endothelial Cells, Cancer, Cell migration, medicine.disease, Coculture Techniques, Gene Expression Regulation, Neoplastic, medicine.anatomical_structure, Oncology, Cancer cell, Disease Progression, Cancer research, sense organs, tumour microenvironment |
| الوصف: | Background: Tumour cells and stromal cells interact in the tumour microenvironment; moreover, stromal cells can acquire abnormalities that contribute to tumour progression. However, interactions between lymphatic endothelial cells (LECs) and tumour cells are largely unexamined. In this study, we aimed to determine whether tumour-specific LECs inhabit the tumour microenvironment and examine their influence on this microenvironment. Methods: We isolated normal LECs (NLECs) from a non-metastatic lymph node and tumour-associated LECs (TLECs) from cancerous lymph nodes. We examined proliferative and migratory potency, growth factor production, and gene expression of each type of LEC. Moreover, we developed a co-culture system to investigate the interactions between gastric cancer cells and LECs. Results: When compared with NLEC, TLECs had an abnormal shape, high proliferative and migratory abilities, and elevated expression of genes associated with inflammation, cell growth, and cell migration. NLECs co-cultured with gastric cancer cells from the OCUM12 cell line acquired TLEC-like phenotypes. Also, OCUM12 cells co-cultured with TLECs expressed high levels of genes responsible for metastasis. Conclusions: Our results demonstrated that LECs interacted with tumour cells and obtained abnormal phenotypes that could have important roles in tumour progression. |
| تدمد: | 1532-1827 0007-0920 |
| DOI: | 10.1038/bjc.2015.282 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c22214b1be30e61cd93f27d114e151df https://doi.org/10.1038/bjc.2015.282 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....c22214b1be30e61cd93f27d114e151df |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15321827 00070920 |
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| DOI: | 10.1038/bjc.2015.282 |