Revisiting rat spermatogenesis with MALDI imaging at 20-microm resolution
| العنوان: | Revisiting rat spermatogenesis with MALDI imaging at 20-microm resolution |
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| المؤلفون: | Michael Becker, Régis Lavigne, Soeren-Oliver Deininger, Detlev Suckau, Mélanie Lagarrigue, Charles Pineau, Florence Aubry, Axel Walch |
| المساهمون: | Swiss Institute of Bioinformatics [Lausanne] (SIB), Université de Lausanne (UNIL), Groupe d'Etude de la Reproduction Chez l'Homme et les Mammiferes (GERHM), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES), Bruker Daltonik GmbH, Bruker Daltonik, Université de Lausanne = University of Lausanne (UNIL), Université de Rennes (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM) |
| المصدر: | Mol. Cell. Proteomics 10:M110.005991 (2011) Molecular and Cellular Proteomics Molecular and Cellular Proteomics, American Society for Biochemistry and Molecular Biology, 2011, 10 (3), pp.M110.005991. ⟨10.1074/mcp.M110.005991⟩ Molecular and Cellular Proteomics, 2011, 10 (3), pp.M110.005991. ⟨10.1074/mcp.M110.005991⟩ |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | MALDI imaging, Male, MESH: Amino Acid Sequence, MESH: Rats, Sprague-Dawley, 01 natural sciences, Biochemistry, Analytical Chemistry, Rats, Sprague-Dawley, MESH: Animals, MESH: Proteins, Biomarker discovery, Image resolution, 0303 health sciences, Chemistry, CELL DEBRIS, Seminiferous Tubules, Immunohistochemistry, Molecular Imaging, MESH: Reproducibility of Results, Protein Transport, MESH: Lasers, Multivariate statistical, MESH: Spermatogenesis, Imaging mass spectrometry, spermatogenesis, testis, MALDI, time-of-flight, MESH: Protein Transport, MESH: Rats, MESH: Seminiferous Tubules, Molecular Sequence Data, Nanotechnology, Mass spectrometry, Models, Biological, 03 medical and health sciences, Animals, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Amino Acid Sequence, Spermatogenesis, Molecular Biology, 030304 developmental biology, MESH: Molecular Sequence Data, MESH: Molecular Imaging, Lasers, Research, 010401 analytical chemistry, MESH: Models, Biological, Proteins, Reproducibility of Results, MESH: Immunohistochemistry, MESH: Male, 0104 chemical sciences, Rats, MESH: Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, High definition, Molecular imaging, Biomedical engineering |
| الوصف: | International audience; Matrix-assisted laser desorption/ionization (MALDI) molecular imaging technology attracts increasing attention in the field of biomarker discovery. The unambiguous correlation between histopathology and MALDI images is a key feature for success. MALDI imaging mass spectrometry (MS) at high definition thus calls for technological developments that were established by a number of small steps. These included tissue and matrix preparation steps, dedicated lasers for MALDI imaging, an increase of the robustness against cell debris and matrix sublimation, software for precision matching of molecular and microscopic images, and the analysis of MALDI imaging data using multivariate statistical methods. The goal of these developments is to approach single cell resolution with imaging MS. Currently, a performance level of 20-μm image resolution was achieved with an unmodified and commercially available instrument for proteins detected in the 2-16-kDa range. The rat testis was used as a relevant model for validating and optimizing our technological developments. Indeed, testicular anatomy is among the most complex found in mammalian bodies. In the present study, we were able to visualize, at 20-μm image resolution level, different stages of germ cell development in testicular seminiferous tubules; to provide a molecular correlate for its well established stage-specific classification; and to identify proteins of interest using a top-down approach and superimpose molecular and immunohistochemistry images. |
| وصف الملف: | application/pdf |
| تدمد: | 1535-9484 1535-9476 |
| DOI: | 10.1074/mcp.M110.005991⟩ |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c1bc013da3f857ed8e9d83042e05ebe4 https://pubmed.ncbi.nlm.nih.gov/21149303 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....c1bc013da3f857ed8e9d83042e05ebe4 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15359484 15359476 |
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| DOI: | 10.1074/mcp.M110.005991⟩ |