A Rapid, Multiplexed, High-Throughput Flow-Through Membrane Immunoassay: A Convenient Alternative to ELISA
| العنوان: | A Rapid, Multiplexed, High-Throughput Flow-Through Membrane Immunoassay: A Convenient Alternative to ELISA |
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| المؤلفون: | Paul Yager, Sujatha Ramachandran, Amit Arjyal, Jeremy Farrar, Gonzalo J. Domingo, Stephen Baker, Buddha Basnyat, Barry R. Lutz, Sabina Dongol, Mitra Singhal, Katherine G. McKenzie, Christiane Dolecek, Jennifer L. Osborn |
| المصدر: | Diagnostics Diagnostics; Volume 3; Issue 2; Pages: 244-260 Diagnostics, Vol 3, Iss 2, Pp 244-260 (2013) |
| بيانات النشر: | MDPI, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Clinical Biochemistry, 01 natural sciences, Multiplexing, Article, 03 medical and health sciences, chemistry.chemical_compound, medicine, High surface area, Multiplex, enzyme-linked immunosorbent assay (ELISA), indirect IgM assay, Throughput (business), 030304 developmental biology, serodiagnosis, 0303 health sciences, lcsh:R5-920, Chromatography, flow-through membrane immunoassay (FMIA), multiplex, Salmonella enterica serovar Typhi, typhoid, low resource setting, medicine.diagnostic_test, Chemistry, 010401 analytical chemistry, Molecular biology, 0104 chemical sciences, Dry storage, Membrane, Immunoassay, lcsh:Medicine (General), Nitrocellulose |
| الوصف: | This paper describes a rapid, high-throughput flow-through membrane immunoassay (FMIA) platform. A nitrocellulose membrane was spotted in an array format with multiple capture and control reagents for each sample detection area, and assay steps were carried out by sequential aspiration of sample and reagents through each detection area using a 96-well vacuum manifold. The FMIA provides an alternate assay format with several advantages over ELISA. The high surface area of the membrane permits high label concentration using gold labels, and the small pores and vacuum control provide rapid diffusion to reduce total assay time to ~30 min. All reagents used in the FMIA are compatible with dry storage without refrigeration. The results appear as colored spots on the membrane that can be quantified using a flatbed scanner. We demonstrate the platform for detection of IgM specific to lipopolysaccharides (LPS) derived from Salmonella Typhi. The FMIA format provides analytical results comparable to ELISA in less time, provides integrated assay controls, and allows compensation for specimen-to-specimen variability in background, which is a particular challenge for IgM assays. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2075-4418 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c029d9a3596711e3245d8ceabe66b767 http://europepmc.org/articles/PMC4665536 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....c029d9a3596711e3245d8ceabe66b767 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20754418 |
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