Growth of Chlamydia trachomatis in enucleated cells
| العنوان: | Growth of Chlamydia trachomatis in enucleated cells |
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| المؤلفون: | E Perara, T S Yen, D Ganem |
| المصدر: | Infection and Immunity. 58:3816-3818 |
| بيانات النشر: | American Society for Microbiology, 1990. |
| سنة النشر: | 1990 |
| مصطلحات موضوعية: | Cytoplasm, Cytoplasmic inclusion, Immunology, Chlamydiae, Chlamydia trachomatis, medicine.disease_cause, Microbiology, Mice, chemistry.chemical_compound, medicine, Animals, Chlamydiaceae, Cytochalasin, Cells, Cultured, Cell Nucleus, biology, Intracellular parasite, Chlamydia Infections, biology.organism_classification, Virology, Cell nucleus, Infectious Diseases, medicine.anatomical_structure, chemistry, Chlamydiales, Parasitology, Research Article |
| الوصف: | Chlamydia trachomatis is an obligate intracellular parasite of eucaryotic cells. Little is known about the role of the host in supporting chlamydial replication beyond the facts that host cells provide ATP and that de novo host protein synthesis is not required for bacterial growth. To further explore potential contributions of host nuclear function to chlamydial development, we questioned whether murine C. trachomatis could grow in mouse L cells that had been enucleated with cytochalasin B. Following enucleation, cells were infected with chlamydiae and analyzed morphologically and biochemically. Late in infection, substantial numbers of chlamydiae of all developmental stages were seen within large cytoplasmic inclusions that were indistinguishable from those seen in infected intact cells. Normal numbers of infectious progeny particles were produced from enucleated cultures. We conclude that active host cell nuclear function is not required to support the growth of chlamydiae. |
| تدمد: | 1098-5522 0019-9567 |
| DOI: | 10.1128/iai.58.11.3816-3818.1990 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bf6e5dafe8dbb5d34a1eac2d069974ff https://doi.org/10.1128/iai.58.11.3816-3818.1990 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....bf6e5dafe8dbb5d34a1eac2d069974ff |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10985522 00199567 |
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| DOI: | 10.1128/iai.58.11.3816-3818.1990 |