A new method to monitor internal training load from muscle oxygen saturation using near-infrared spectroscopy could be of practical application for research and training purposes. This technology has been validated in different scientific fields, including sports science, and Humon Hex and Moxy are two leading brands. However, its relationship with hemoglobin has not been studied. Forty-eight professional cyclists, 19 men and 29 women, underwent a blood test to measure hemoglobin in the early morning. Immediately afterwards, hemoglobin and muscle oxygenation were monitored at rest by Moxy and Humon Hex on their right quadriceps (where the skinfold was measured). Venous blood hemoglobin was higher than the measurement for both devices (p