التفاصيل البيبلوغرافية
| العنوان: |
Mechanism of Activation for Transcription Factor PhoB Suggested by Different Modes of Dimerization in the Inactive and Active States |
| المؤلفون: |
G. V. T. Swapna, Priti Bachhawat, Ann M. Stock, Gaetano T. Montelione |
| المصدر: |
Structure. (9):1353-1363 |
| بيانات النشر: |
Elsevier Ltd. |
| مصطلحات موضوعية: |
inorganic chemicals, Materials science, Protein Structure, Secondary, Article, Dephosphorylation, 03 medical and health sciences, chemistry.chemical_compound, Bacterial Proteins, Structural Biology, Direct repeat, Phosphorylation, Binding site, Molecular Biology, Transcription factor, 030304 developmental biology, 0303 health sciences, Binding Sites, Crystallography, 030306 microbiology, Effector, Escherichia coli Proteins, DNA-binding domain, Solutions, Biochemistry, chemistry, Biophysics, Dimerization, DNA, Transcription Factors |
| الوصف: |
Response regulators (RRs), which undergo phosphorylation/dephosphorylation at aspartate residues, are highly prevalent in bacterial signal transduction. RRs typically contain an N-terminal receiver domain that regulates the activities of a C-terminal DNA-binding domain in a phosphorylation-dependent manner. We present crystallography and solution NMR data for the receiver domain of Escherichia coli PhoB, which show distinct two-fold symmetric dimers in the inactive and active states, providing the first such pair within the OmpR/PhoB subfamily, the largest group of RRs. These structures together with the previously determined structure of the C-terminal domain of PhoB bound to DNA, define the conformation of the active transcription factor, and provide a model for the mechanism of activation in this subfamily. In the active state, the receiver domains dimerize with two-fold rotational symmetry using their α4-β5-α5 faces, while the effector domains bind to DNA direct repeats with tandem symmetry, implying a loss of intramolecular interactions. |
| اللغة: |
English |
| تدمد: |
0969-2126 |
| DOI: |
10.1016/j.str.2005.06.006 |
| URL الوصول: |
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::be3e28bf6253115caa2c6819d69a3c71 |
| Rights: |
OPEN |
| رقم الانضمام: |
edsair.doi.dedup.....be3e28bf6253115caa2c6819d69a3c71 |
| قاعدة البيانات: |
OpenAIRE |