Nanocolloidal albumin-IRDye 800CW: a near-infrared fluorescent tracer with optimal retention in the sentinel lymph node

التفاصيل البيبلوغرافية
العنوان: Nanocolloidal albumin-IRDye 800CW: a near-infrared fluorescent tracer with optimal retention in the sentinel lymph node
المؤلفون: Johannes F. de Boer, Gijs J.L. Wuite, C. René Leemans, Gerard W. M. Visser, Guus A.M.S. van Dongen, Wouter H. Roos, Derrek A. Heuveling, Marian Baclayon, Mattijs de Groot, Remco de Bree
المساهمون: Physics of Living Systems, Biophotonics and Medical Imaging, LaserLaB - Molecular Biophysics, LaserLaB - Biophotonics and Microscopy, Otolaryngology / Head & Neck Surgery, Radiology and nuclear medicine, CCA - Disease profiling, Molecular Biophysics
المصدر: European Journal of Nuclear Medicine and Molecular Imaging, 39(7), 1161-1168. Springer Verlag
European Journal of Nuclear Medicine and Molecular Imaging, 39(7), 1161-1168. SPRINGER
Heuveling, D A, Visser, G W M, de Groot, M, de Boer, J F, Salumbides-Baclayon, M, Roos, W H, Wuite, G J L, Leemans, C R, de Bree, R & van Dongen, G A M S 2012, ' Nanocolloidal albumin-IRDye 800CW: a near-infrared fluorescent tracer with optimal retention in the sentinel lymph node ', European Journal of Nuclear Medicine and Molecular Imaging, vol. 39, no. 7, pp. 1161-1168 . https://doi.org/10.1007/s00259-012-2080-5
European Journal of Nuclear Medicine and Molecular Imaging
سنة النشر: 2012
مصطلحات موضوعية: Fluorescence-lifetime imaging microscopy, Pathology, IRDye 800CW, Indoles, Skin Neoplasms, indocyanine green, Near-infrared fluorescence, chemistry.chemical_compound, sentinel lymph node, Fluorescent tracer, Spectroscopy, Near-Infrared, Chemistry, Benzenesulfonates, article, General Medicine, Sentinel node, tracer, particle size, unclassified drug, Radiology Nuclear Medicine and imaging, Lymphatic Metastasis, Original Article, Female, Rabbits, medicine.medical_specialty, Sentinel lymph node, serum albumin, animal experiment, rabbit, lymph node dissection, fluorescence imaging, TRACER, medicine, Animals, Radiology, Nuclear Medicine and imaging, controlled study, Nanocolloidal albumin, Radionuclide Imaging, Technetium Tc 99m Aggregated Albumin, Fluorescent Dyes, ICG, nonhuman, nanocolloidal albumin irdye 800cw, Sentinel Lymph Node Biopsy, animal model, Near-infrared spectroscopy, Radiochemistry, Albumin, Radiography, body regions, Disease Models, Animal, good manufacturing practice, Lymph Node Excision, Lymph Nodes, Radiopharmaceuticals, quantum yield, Indocyanine green
الوصف: Purpose At present, the only approved fluorescent tracer for clinical near-infrared fluorescence-guided sentinel node (SN) detection is indocyanine green (ICG), but the use of this tracer is limited due to its poor retention in the SN resulting in the detection of higher tier nodes. We describe the development and characterization of a next-generation fluorescent tracer, nanocolloidal albumin-IRDye 800CW that has optimal properties for clinical SN detection Methods The fluorescent dye IRDye 800CW was covalently coupled to colloidal human serum albumin (HSA) particles present in the labelling kit Nanocoll in a manner compliant with current Good Manufacturing Practice. Characterization of nanocolloidal albumin-IRDye 800CW included determination of conjugation efficiency, purity, stability and particle size. Quantum yield was determined in serum and compared to that of ICG. For in vivo evaluation a lymphogenic metastatic tumour model in rabbits was used. Fluorescence imaging was performed directly after peritumoral injection of nanocolloidal albumin-IRDye 800CW or the reference ICG/HSA (i.e. ICG mixed with HSA), and was repeated after 24 h, after which fluorescent lymph nodes were excised. Results Conjugation of IRDye 800CW to nanocolloidal albumin was always about 50% efficient and resulted in a stable and pure product without affecting the particle size of the nanocolloidal albumin. The quantum yield of nanocolloidal albumin-IRDye 800CW was similar to that of ICG. In vivo evaluation revealed noninvasive detection of the SN within 5 min of injection of either nanocolloidal albumin-IRDye 800CW or ICG/HSA. No decrease in the fluorescence signal from SN was observed 24 h after injection of the nanocolloidal albumin-IRDye 800CW, while a strong decrease or complete disappearance of the fluorescence signal was seen 24 h after injection of ICG/HSA. Fluorescence-guided SN biopsy was very easy. Conclusion Nanocolloidal albumin-IRDye 800CW is a promising fluorescent tracer with optimal kinetic features for SN detection. Electronic supplementary material The online version of this article (doi:10.1007/s00259-012-2080-5) contains supplementary material, which is available to authorized users.
وصف الملف: application/pdf
اللغة: English
تدمد: 1619-7070
DOI: 10.1007/s00259-012-2080-5
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bdf2bded1955473e682c42204200e789
https://doi.org/10.1007/s00259-012-2080-5
Rights: OPEN
رقم الانضمام: edsair.doi.dedup.....bdf2bded1955473e682c42204200e789
قاعدة البيانات: OpenAIRE
الوصف
تدمد:16197070
DOI:10.1007/s00259-012-2080-5