Polymer-coated albumin microspheres as carriers for intravascular tumour targeting of cisplatin

التفاصيل البيبلوغرافية
العنوان: Polymer-coated albumin microspheres as carriers for intravascular tumour targeting of cisplatin
المؤلفون: R. Verrijk, J. G. McVie, Adrian C. Begg, I. J. H. Smolders
المصدر: Cancer chemotherapy and pharmacology. 29(2)
سنة النشر: 1991
مصطلحات موضوعية: inorganic chemicals, Cancer Research, Stereochemistry, Drug Compounding, Melanoma, Experimental, Pharmacology, Toxicology, Dosage form, medicine, Animals, Pharmacology (medical), Cytotoxicity, Leukemia L1210, neoplasms, Tumor Stem Cell Assay, Cisplatin, Chemistry, Albumin, female genital diseases and pregnancy complications, In vitro, Microspheres, Oncology, Cell culture, Delayed-Action Preparations, Liberation, L1210 cells, medicine.drug
الوصف: We used a poly-lactide-co-glycolide polymer (PLAGA 50:50) to formulate cisplatin (cDDP) into microspheres designed for intravascular administration. Two systems were developed. PLAGA-coated albumin microspheres and microspheres consisting of PLAGA only. PLAGA-coated microspheres displayed a mean diameter of 31.8 +/- 0.9 microns and a payload of 7.5% cDDP (w/w). Solid PLAGA microspheres exhibited a mean diameter of 19.4 +/- 0.6 microns and a payload of 20% cDDP. Release characteristics and in vitro effects on L1210 leukemia and B16 melanoma cell lines were investigated. Both types of microsphere overcame the initial rapid release of cDDP (burst effect), and PLAGA-coated albumin microspheres also showed a lag phase of approximately 30 min before cDDP release began. PLAGA-coated albumin microspheres released most of their payload through diffusion, and the coating eventually cracked after 7 days' incubation in saline supplemented with 0.1% Tween at 37 degrees C, enabling the release of any cDDP remaining. Effects of platinum, pre-released from PLAGA-coated albumin microspheres on the in vitro growth of L1210 cells were comparable with those of standard formulations (dissolved) of cDDP. Material released from non-drug-loaded PLAGA microspheres had no effect on L1210 cell growth, suggesting the absence of cytotoxic compounds in the matrix. The colony-forming ability of B16 cells was also equally inhibited by standard cDDP and pre-released drug. These studies show that formulation of cDDP in PLAGA-based microspheres prevents the rapid burst effect of cDDP seen in previous preparations and offers an improved system of administration for hepatic artery infusion or adjuvant therapy, enabling better clinical handling and the promise of a higher ratio of tumour tissue to normal tissue.
تدمد: 0344-5704
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bdd14fcd7cc214c6d030461ecea3d230
https://pubmed.ncbi.nlm.nih.gov/1760853
Rights: CLOSED
رقم الانضمام: edsair.doi.dedup.....bdd14fcd7cc214c6d030461ecea3d230
قاعدة البيانات: OpenAIRE