Targeted genetic testing for familial hypercholesterolaemia using next generation sequencing:a population-based study
| العنوان: | Targeted genetic testing for familial hypercholesterolaemia using next generation sequencing:a population-based study |
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| المؤلفون: | Blair H. Smith, Archie Campbell, Ellen R A Thomas, David J. Porteous, Laurence Game, Shona M. Kerr, Andrew D. Morris, Anne K. Soutar, Jennifer Biggs, Timothy J. Aitman, Jana Vandrovcova, Penny J. Norsworthy, Generation Scotland, Anna F. Dominiczak |
| المصدر: | 2014, ' Targeted genetic testing for familial hypercholesterolaemia using next generation sequencing : a population-based study ', BMC Medical Genetics, vol. 15, no. 1, pp. 70 . https://doi.org/10.1186/1471-2350-15-70 BMC Medical Genetics |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | Adult, Male, medicine.medical_specialty, Population, DNA Mutational Analysis, Biology, Bioinformatics, High cholesterol, Hyperlipoproteinemia Type II, Internal medicine, medicine, Genetics, Molecular diagnostic testing, Humans, Clinical significance, Genetics(clinical), Multiplex ligation-dependent probe amplification, Genetic Testing, Total cholesterol, education, Genetics (clinical), Genetic testing, Aged, education.field_of_study, medicine.diagnostic_test, PCSK9, Serine Endopeptidases, High-Throughput Nucleotide Sequencing, Middle Aged, medicine.disease, Primary care, Human genetics, 3. Good health, LDLR, Receptors, LDL, Scotland, Mutation (genetic algorithm), Mutation, Next-generation sequencing, Female, Proprotein Convertases, Proprotein Convertase 9, Familial hypercholesterolaemia, Generation Scotland, Research Article |
| الوصف: | Background\ud Familial hypercholesterolaemia (FH) is a common Mendelian condition which, untreated, results in premature coronary heart disease. An estimated 88% of FH cases are undiagnosed in the UK. We previously validated a method for FH mutation detection in a lipid clinic population using next generation sequencing (NGS), but this did not address the challenge of identifying index cases in primary care where most undiagnosed patients receive healthcare. Here, we evaluate the targeted use of NGS as a potential route to diagnosis of FH in a primary care population subset selected for hypercholesterolaemia.\ud \ud Methods\ud We used microfluidics-based PCR amplification coupled with NGS and multiplex ligation-dependent probe amplification (MLPA) to detect mutations in LDLR, APOB and PCSK9 in three phenotypic groups within the Generation Scotland: Scottish Family Health Study including 193 individuals with high total cholesterol, 232 with moderately high total cholesterol despite cholesterol-lowering therapy, and 192 normocholesterolaemic controls.\ud \ud Results\ud Pathogenic mutations were found in 2.1% of hypercholesterolaemic individuals, in 2.2% of subjects on cholesterol-lowering therapy and in 42% of their available first-degree relatives. In addition, variants of uncertain clinical significance (VUCS) were detected in 1.4% of the hypercholesterolaemic and cholesterol-lowering therapy groups. No pathogenic variants or VUCS were detected in controls.\ud \ud Conclusions\ud We demonstrated that population-based genetic testing using these protocols is able to deliver definitive molecular diagnoses of FH in individuals with high cholesterol or on cholesterol-lowering therapy. The lower cost and labour associated with NGS-based testing may increase the attractiveness of a population-based approach to FH detection compared to genetic testing with conventional sequencing. This could provide one route to increasing the present low percentage of FH cases with a genetic diagnosis. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1471-2350 |
| DOI: | 10.1186/1471-2350-15-70 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bd617e9cb272eb8500acffe835936200 https://hdl.handle.net/20.500.11820/fdd55486-81f2-4f09-a70b-b36e4dc1dbd2 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....bd617e9cb272eb8500acffe835936200 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14712350 |
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| DOI: | 10.1186/1471-2350-15-70 |