Antimicrobial activity of bovine β-lactoglobulin against mastitis-causing bacteria
| العنوان: | Antimicrobial activity of bovine β-lactoglobulin against mastitis-causing bacteria |
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| المؤلفون: | L. Chaneton, J.M. Pérez Sáez, Leonardo E. Bussmann |
| المصدر: | CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | Staphylococcus aureus, Otras Ciencias Biológicas, Colony Count, Microbial, Lactoglobulins, medicine.disease_cause, Microbiology, Ciencias Biológicas, purl.org/becyt/ford/1 [https], Anti-Infective Agents, Genetics, medicine, Escherichia coli, Animals, ANTIMICROBIAL, purl.org/becyt/ford/1.6 [https], Mastitis, Bovine, Streptococcus uberis, biology, Streptococcus, Lactoferrin, biology.organism_classification, medicine.disease, Antimicrobial, Mastitis, biology.protein, Animal Science and Zoology, Cattle, Female, CIENCIAS NATURALES Y EXACTAS, Bacteria, MASTITIS-CAUSING BACTERIA, Food Science, Β-LACTOGLOBULIN |
| الوصف: | Bovine mastitis is one of the most economically deleterious diseases affecting dairy herds and results from an infection of the udder by pathogenic microorganisms such as Staphylococcus aureus, Streptococcus uberis, and Escherichia coli. The mammary gland is capable of preventing and combating bacterial infection by means of a complex network of innate and adaptive immune mechanisms. Lactoferrin is an 86-kDa protein with antibacterial activity that plays a role in the mammary gland's defense against infection. β-Lactoglobulin (β-LG) is an 18-kDa protein that is present in most mammals but is notably absent in humans, rodents, and lagomorphs. Different genetic variants of this protein exist, with β-LG A and β-LG B being the most common. In spite of being well studied, the biological function of β-LG is not thoroughly understood, and most noticeably, no reports exist on the effects of the native protein on bacterial growth. Hence, the objective of this study was to assess the potential antibacterial activity of β-LG against mastitis agents. To do this, we purified β-LG from normal bovine milk using a mild, nondenaturing method and performed in vitro growth inhibition assays with Staph. aureus, E. coli, and Strep. uberis. β-Lactoglobulin inhibited the growth of Staph. aureus and Strep. uberis but had no effect on E. coli. The antimicrobial activity against Staph. aureus and Strep. uberis was concentration dependent and was elicited by the intact protein because Tricine-sodium dodecyl sulfate-PAGE and analytical gel filtration chromatography did not reveal the presence of short degradation peptides. Analysis of the genetic variants of β-LG showed that β-LG A has higher inhibitory activity against Staph. aureus and Strep. uberis than β-LG B. Coincubation of β-LG and lactoferrin resulted in an augmented antibacterial activity against Staph. aureus, suggesting an additive effect of the proteins. This result, along with the proteins' complementary spectrum of action, suggests that β-LG and lactoferrin may complement each other in the mammary gland's defenses against bacterial infection. © 2011 American Dairy Science Association. Fil: Chaneton, Luciano. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina Fil: Pérez Sáez, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina Fil: Bussmann, Leonardo Edmundo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina |
| وصف الملف: | application/pdf |
| تدمد: | 1525-3198 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bd5d564fac9f1c6f5862a8ed2b04c2f8 https://pubmed.ncbi.nlm.nih.gov/21183025 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....bd5d564fac9f1c6f5862a8ed2b04c2f8 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15253198 |
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