Selective expression of KCNA5 and KCNB1 genes in gastric and colorectal carcinoma
| العنوان: | Selective expression of KCNA5 and KCNB1 genes in gastric and colorectal carcinoma |
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| المؤلفون: | Mouadh Barbirou, Mousadak Azzouz Mohamed, Mouna Madhioub, Amira Jaballah Gabteni, Balkiss Bouhaouala-Zahar, Salsabil Atafi, Asma Ouakaa Kchaou, Lamine Hamzaoui, Azer Farah, Emna Chelbi, Hassen Touinsi, Maria Kabbage, Rahma Ben Abderrazek, Samir Boubaker |
| المصدر: | BMC Cancer BMC Cancer, Vol 20, Iss 1, Pp 1-9 (2020) |
| بيانات النشر: | BioMed Central, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Male, Cancer Research, Colorectal cancer, Biology, lcsh:RC254-282, 03 medical and health sciences, Kv1.5 Potassium Channel, 0302 clinical medicine, Shab Potassium Channels, Stomach Neoplasms, Gene expression, Genetics, medicine, Humans, Retrospective Studies, Kv1.5, Kv2.1, Cancer, Cancer diagnosis, Middle Aged, medicine.disease, lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens, Early Gastric Cancer, 030104 developmental biology, Real-time polymerase chain reaction, Oncology, 030220 oncology & carcinogenesis, Cancer cell, Cancer research, Immunohistochemistry, Female, Gastric cancer, Colorectal Neoplasms, Intracellular localisation, Immunostaining, Research Article |
| الوصف: | Background Gastric and colorectal cancers are the most common malignant tumours, leading to a significant number of cancer-related deaths worldwide. Recently, increasing evidence has demonstrated that cancer cells exhibit a differential expression of potassium channels and this can contribute to cancer progression. However, their expression and localisation at the somatic level remains uncertain. In this study, we have investigated the expression levels of KCNB1 and KCNA5 genes encoding ubiquitous Kv2.1 and Kv1.5 potassium channels in gastric and colorectal tumours. Methods Gastric and colorectal tumoral and peritumoral tissues were collected to evaluate the expression of KCNB1 and KCNA5 mRNA by quantitative PCR. Moreover, the immunohistochemical staining profile of Kv2.1 and Kv1.5 was assessed on 40 Formalin-Fixed and Paraffin-Embedded (FFPE) gastric carcinoma tissues. Differences in gene expression between tumoral and peritumoral tissues were compared statistically with the Mann-Whitney U test. The association between the clinicopathological features of the GC patients and the expression of both Kv proteins was investigated with χ2 and Fisher’s exact tests. Results The mRNA fold expression of KCNB1 and KCNA5 genes showed a lower mean in the tumoral tissues (0.06 ± 0.17, 0.006 ± 0.009) compared to peritumoral tissues (0.08 ± 0.16, 0.16 ± 0.48, respectively) without reaching the significance rate (p = 0.861, p = 0.152, respectively). Interestingly, Kv2.1 and Kv1.5 immunostaining was detectable and characterised by a large distribution in peritumoral and tumoral epithelial cells. More interestingly, inflammatory cells were also stained. Surprisingly, Kv2.1 and Kv1.5 staining was undoubtedly and predominantly detected in the cytoplasm compartment of tumour cells. Indeed, the expression of Kv2.1 in tumour cells revealed a significant association with the early gastric cancer clinical stage (p = 0.026). Conclusion The data highlight, for the first time, the potential role of Kv1.5 and Kv2.1 in gastrointestinal-related cancers and suggests they may be promising prognostic markers for these tumours. |
| اللغة: | English |
| تدمد: | 1471-2407 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bccdf02345c4438503ae76490c81f70e http://europepmc.org/articles/PMC7709444 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....bccdf02345c4438503ae76490c81f70e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14712407 |
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