Arabidopsis P-Protein Filament Formation Requires Both AtSEOR1 and AtSEOR2
| العنوان: | Arabidopsis P-Protein Filament Formation Requires Both AtSEOR1 and AtSEOR2 |
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| المؤلفون: | Gary A. Thompson, Michael Knoblauch, James A. Anstead, Daniel R. Froelich |
| المصدر: | Plant and Cell Physiology. 53:1033-1042 |
| بيانات النشر: | Oxford University Press (OUP), 2012. |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | DNA, Bacterial, Physiology, Recombinant Fusion Proteins, Green Fluorescent Proteins, Mutant, Arabidopsis, Plant Science, Phloem, Genes, Plant, Dithiothreitol, Host-Parasite Interactions, Green fluorescent protein, Protein filament, Open Reading Frames, Structure-Activity Relationship, chemistry.chemical_compound, Gene Expression Regulation, Plant, Animals, Herbivory, RNA, Messenger, Sequence Homology, Amino Acid, biology, Arabidopsis Proteins, Endoplasmic reticulum, Genetic Complementation Test, fungi, Antibodies, Monoclonal, food and beverages, Cell Biology, General Medicine, Plants, Genetically Modified, biology.organism_classification, Mutagenesis, Insertional, Open reading frame, Biochemistry, chemistry, Aphids, Plasmids |
| الوصف: | The structure–function relationship of proteinaceousfilaments in sieve elements has long been a source of inves-tigation in order to understand their role in the biology ofthe phloem. Two phloem filament proteins AtSEOR1(At3g01680.1) and AtSEOR2 (At3g01670.1) in Arabidopsishave been identified that are required for filament forma-tion. Immunolocalization experiments using a phloemfilament-specific monoclonal antibody in the respectiveT-DNA insertion mutants provided an initial indicationthat both proteins are necessary to form phloem filaments.To investigate the relationship between these two proteinsfurther, green fluorescent protein (GFP)–AtSEO fusionproteins were expressed in Columbia wild-type and T-DNAinsertion mutants. Analysis of these mutants by confocalmicroscopy confirmed that phloem filaments could onlybe detected in the presence of both proteins, indicatingthat despite significant sequence homology the proteinsare not functionally redundant. Individual phloem filamentprotein subunits of AtSEOR1 and AtSEOR2 were capable offorming homodimers, but not heterodimers in a yeasttwo-hybrid system. The absence of phloem filaments inphloem sieve elements did not result in gross alterationsof plant phenotype or affect basal resistance to greenpeach aphid (Myzus persicae).Keywords: Arabidopsis AtSEOR Myzus persicae Phloem P-protein Sieve element.Abbreviations: DTT, dithiothreitol; ER, endoplasmic reticu-lum; GFP, green fluorescent protein; MALDI-TOF MS,matrix-assisted laser desorption ionization-time of flightmass spectrometry; ORF, open reading frame; PBS, phos-phate-buffered saline; P-protein, phloem protein; RT–PCR,reverse transcription–PCR; SE, sieve element; SEO, sieve elem-ent occlusion protein; SEOR, sieve element occlusion-relatedprotein; YFP, yellow fluorescent protein. |
| تدمد: | 1471-9053 0032-0781 |
| DOI: | 10.1093/pcp/pcs046 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ba6dc2e4a4ce8085e47d55c8c8c1b827 https://doi.org/10.1093/pcp/pcs046 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....ba6dc2e4a4ce8085e47d55c8c8c1b827 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14719053 00320781 |
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| DOI: | 10.1093/pcp/pcs046 |