In vitro regeneration and flowering of Portulaca grandiflora Hook
| العنوان: | In vitro regeneration and flowering of Portulaca grandiflora Hook |
|---|---|
| المؤلفون: | Claudinei da Silva Souza, Diego Ismael Rocha, Carla Fernandes Cruz, Ilio Fealho de Carvalho, Maurecilne Lemes da Silva, Wolffe Ferreira dos Santos, Marcelo Dias Machado |
| المصدر: | Ornamental Horticulture, Vol 25, Iss 4, Pp 443-449 (2019) Ornamental Horticulture, Volume: 25, Issue: 4, Pages: 443-449, Published: 24 JAN 2020 Ornamental Horticulture v.25 n.4 2019 Ornamental Horticulture (Campinas) Sociedade Brasileira de Floricultura e Plantas Ornamentais (SBFPO) instacron:SBFPO |
| بيانات النشر: | Sociedade Brasileira de Floricultura e Plantas Ornamentais, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0106 biological sciences, Plant Science, Horticulture, lcsh:Plant culture, 01 natural sciences, chemistry.chemical_compound, Shoot organogenesis, Anthesis, Ornamental plant, lcsh:SB1-1110, Gibberellic acid, Ácido giberélico, biology, fungi, food and beverages, in vitro flowering, gibberellic acid, shoot organogenesis, 04 agricultural and veterinary sciences, In vitro Flowering, organogênese, biology.organism_classification, chemistry, Portulaca grandiflora, Florescimento in vitro, Shoot, 040103 agronomy & agriculture, 0401 agriculture, forestry, and fisheries, Petal, Kinetin, 010606 plant biology & botany, Explant culture |
| الوصف: | P. grandiflora is a known ornamental plant with abundant flowering. The flowers exhibit varied coloration with distinct forms and simple folded petals and/or multiple. The objective of this work was to induce regeneration via organogenesis and in vitro flowering of P. grandiflora. Nodal segments of seedlings germinated in vitro were used as explant source for regeneration. Kinetin (KIN) and 6-Benzylaminopurine (BA) were used for the induction of organogenesis. The treatments supplemented with 1.0 and 1.5 mg L−1 BA induced the highest number of adventitious shoots with an average number of 7.0 (±1.55) e 5.4 (±0.83), respectively. The microcuttings obtained from regenerated shoots produced floral buds. The floral buds were located in the axillary and terminal regions of the microcuttings and developed in approximately 10 days of cultivation until the anthesis. The highest number of flower buds was observed in the presence of 0.75 mg L−1 of gibberellic acid. This study opens new perspectives for the establishment of biotechnological tools to be applied for this important ornamental species. Resumo P. grandiflora é uma das plantas ornamentais com abundante floração de coloração variada com formas distintas e pétalas simples, dobradas e/ou múltiplas. O objetivo do trabalho foi induzir a regeneração pela via organogênica e o florescimento in vitro de P. grandiflora. Segmentos nodais de plântulas germinadas in vitro foram utilizados como fonte de explante para a regeneração. Cinetina (KIN) e 6-Benzilaminopurina (BA) foram utilizadas para a indução de organogênese de novo. Os tratamentos suplementados com 1.0, 1.5 mg L−1 de BA induziram a formação de maior número de brotações adventícias com número médio de 7.0 (±1,55) e 5.4 (±0.83), respectivamente. As microestacas obtidas a partir de gemas regeneradas produziram botões florais. Os botões florais localizaram-se nas regiões axilar e terminal das microestacas e desenvolveram-se em aproximadamente 10 dias de cultivo até a antese. O maior número de botões florais foi observado na presença de 0.75 mg L−1 de ácido giberélico. Este estudo abre novas perspectivas para o estabelecimento de ferramentas biotecnológicas a serem aplicadas para essa importante espécie ornamental. |
| وصف الملف: | text/html |
| اللغة: | English |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b9f8a1084117750457d20b0dfb2bf84a https://ornamentalhorticulture.emnuvens.com.br/rbho/article/view/2077 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....b9f8a1084117750457d20b0dfb2bf84a |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |