Site directed confinement of laccases in a porous scaffold towards robustness and selectivity
| العنوان: | Site directed confinement of laccases in a porous scaffold towards robustness and selectivity |
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| المؤلفون: | Jean-Luc Blin, Rénal Backov, Fangfang Yang, Thierry Tron, Yasmina Mekmouche, Bernadett Fáklya |
| المساهمون: | Institut des Sciences Moléculaires de Marseille (ISM2), Aix Marseille Université (AMU)-École Centrale de Marseille (ECM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Centre de Recherche Paul Pascal (CRPP), Université de Bordeaux (UB)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Lorrain de Chimie Moléculaire (L2CM), Institut de Chimie du CNRS (INC)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-École Centrale de Marseille (ECM)-Institut de Chimie du CNRS (INC) |
| المصدر: | Biotechnology Reports Biotechnology Reports, Elsevier, 2021, 31, pp.e00645. ⟨10.1016/j.btre.2021.e00645⟩ Biotechnology Reports, 2021, 31, pp.e00645. ⟨10.1016/j.btre.2021.e00645⟩ Biotechnology Reports, Vol 31, Iss, Pp e00645-(2021) |
| بيانات النشر: | HAL CCSD, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0106 biological sciences, [SDV.BIO]Life Sciences [q-bio]/Biotechnology, Site-directed immobilization, Heterogeneous catalysis, APTES, (3-Aminopropyl)triethoxysilane, 01 natural sciences, Applied Microbiology and Biotechnology, orientation, Catalysis, laccase, 03 medical and health sciences, chemistry.chemical_compound, 010608 biotechnology, Enz., enzyme, TTAB, tetradecyltrimethylammonium bromide, 030304 developmental biology, ComputingMethodologies_COMPUTERGRAPHICS, Laccase, 0303 health sciences, ABTS, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid, S.A., specific activity, Substrate (chemistry), HIPE, High Internal Phase Emulsion, BET, Brunauer, Emmett et Teller, RB5, Reactive black 5, heterogeneous catalysis, chemistry, Chemical engineering, Covalent bond, TNC, TriNuclear Cluster, HBT, N-Hydroxy benzotriazole, Surface modification, TEOS, Tetraethyl-orthosilane, DPBS, Dulbecco's Phosphate-Buffered Saline, pH 7.0, Glutaraldehyde, Asc, ascorbic acid, RBBR, Remazol Brilliant Blue B, Selectivity, TP248.13-248.65, Biotechnology, Research Article |
| الوصف: | Graphical abstract Highlights • Site directed immobilization of laccase on silica foam via covalent bond forming reaction. • RB5 decolorization activity is maintained for 13 reaction cycles or one year storage towards RB5 oxidation. • Active site orientation towards porous material decreases/increases large substrate oxidation. We immobilized a fungal laccase with only two spatially close lysines available for functionalization into macrocellular Si(HIPE) monoliths for the purpose of continuous flow catalysis. Immobilization (30–45 % protein immobilization yields) was obtained using a covalent bond forming reaction between the enzyme and low glutaraldehyde (0.625 % (w/w)) functionalized foams. Testing primarily HBT-mediated RB5 dye decolorization in continuous flow reactors, we show that the activity of the heterogeneous catalyst is comparable to its homogeneous counterpart. More, its operational activity remains as high as 60 % after twelve consecutive decolorization cycles as well as after one-year storage, performances remarkable for such a material. We further immobilized two variants of the laccase containing a unique lysine: one located in the vicinity of the substrate oxidation site (K157) and one at the opposite side of this oxidation site (K71) to study the effect of the proximity of the Si(HIPE) surface on enzyme activity. Comparing activities on different substrates for monoliths with differentially oriented catalysts, we show a twofold discrimination for ABTS relative to ascorbate. This study provides ground for the development of neo-functionalized materials that beyond allowing stability and reusability will become synergic partners in the catalytic process. |
| اللغة: | English |
| تدمد: | 2215-017X |
| DOI: | 10.1016/j.btre.2021.e00645⟩ |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b97de81559f2abee39d1ee29ca3fff55 https://hal.archives-ouvertes.fr/hal-03262272/file/1-s2.0-S2215017X21000618-main.pdf |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....b97de81559f2abee39d1ee29ca3fff55 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 2215017X |
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| DOI: | 10.1016/j.btre.2021.e00645⟩ |