Role of extracellular signal-regulated kinase pathway in RRR-alpha-tocopheryl succinate-induced differentiation of human MDA-MB-435 breast cancer cells

التفاصيل البيبلوغرافية
العنوان: Role of extracellular signal-regulated kinase pathway in RRR-alpha-tocopheryl succinate-induced differentiation of human MDA-MB-435 breast cancer cells
المؤلفون: Kimberly Kline, Weiping Yu, Huihong You, Powel H. Brown, Bob G. Sanders, Debbie Munoz-Medellin
المصدر: Molecular carcinogenesis. 33(4)
سنة النشر: 2002
مصطلحات موضوعية: Cyclin-Dependent Kinase Inhibitor p21, Cancer Research, Mitogen-Activated Protein Kinase 3, MAP Kinase Signaling System, Proto-Oncogene Proteins c-jun, MAP Kinase Kinase 1, Tocopherols, Breast Neoplasms, Mitogen-activated protein kinase kinase, Protein Serine-Threonine Kinases, Transfection, MAP2K7, Oligodeoxyribonucleotides, Antisense, Genes, jun, Cyclins, Tumor Cells, Cultured, Humans, Vitamin E, ASK1, Molecular Biology, MAPK14, Mitogen-Activated Protein Kinase 1, Mitogen-Activated Protein Kinase Kinases, MAP kinase kinase kinase, biology, Cyclin-dependent kinase 4, JNK Mitogen-Activated Protein Kinases, Cell Differentiation, Molecular biology, Recombinant Proteins, Cell biology, Doxycycline, biology.protein, Keratins, Cyclin-dependent kinase 9, Female, Mitogen-Activated Protein Kinases
الوصف: RRR-alpha-tocopheryl succinate (vitamin E succinate, VES) induces differentiation of human breast cancer cells. Previous studies ruled out transforming growth factor-beta and c-jun N-terminal kinase involvement in VES-induced differentiation but implicated extracellular signal-regulated kinases (ERKs). Here we show that dominant-negative mutants of either mitogen-activated protein kinase kinase (MEK) 1 or ERK1 blocked VES-induced differentiation of MDA-MB-435 cells, as measured by induction of cytokeratin 18 and p21 (Waf1/Cip1) proteins. Blockage of c-jun protein expression using c-jun antisense oligonucleotides or expression of an inducible dominant-negative c-jun mutant protein inhibited VES-induced differentiation. Elevated expression of wild-type c-jun alone was sufficient to induce cellular differentiation. A role for p21 (Waf1/Cip1) is implicated, in that p21 antisense oligomers blocked VES-induced differentiation. In summary, MEK1, ERK1, the transcription factor c-jun, and the cyclin-dependent kinase inhibitor p21 (Waf1/Cip1) play a part in VES-induced differentiation of human MDA-MB-435 breast cancer cells.
تدمد: 0899-1987
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b96d38b450f2f8b11807850e609a3be6
https://pubmed.ncbi.nlm.nih.gov/11933076
Rights: CLOSED
رقم الانضمام: edsair.doi.dedup.....b96d38b450f2f8b11807850e609a3be6
قاعدة البيانات: OpenAIRE