Large-Scale Identification of Protein Crotonylation Reveals Its Role in Multiple Cellular Functions
| العنوان: | Large-Scale Identification of Protein Crotonylation Reveals Its Role in Multiple Cellular Functions |
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| المؤلفون: | Xiaoguang Liu, Qiufang Zeng, Lujian Liao, Shennan Gao, Bin Tang, Jiemin Wong, Wei Wei, Jiwen Li, Wenpeng Li, James X. Du, Lu Lu, Anqi Mao |
| المصدر: | Journal of Proteome Research. 16:1743-1752 |
| بيانات النشر: | American Chemical Society (ACS), 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | DNA Replication, Proteomics, 0301 basic medicine, Heterochromatin, Lysine, Cell Cycle Proteins, Biochemistry, Mass Spectrometry, Histones, 03 medical and health sciences, Non-histone protein, Transcription (biology), Gene expression, Humans, Nuclear protein, Promoter Regions, Genetic, biology, General Chemistry, 030104 developmental biology, Histone, Chromobox Protein Homolog 5, biology.protein, Acyl Coenzyme A, Protein Processing, Post-Translational, HeLa Cells |
| الوصف: | Lysine crotonylation on histones is a recently identified post-translational modification that has been demonstrated to associate with active promoters and to directly stimulate transcription. Given that crotonyl-CoA is essential for the acyl transfer reaction and it is a metabolic intermediate widely localized within the cell, we postulate that lysine crotonylation on nonhistone proteins could also widely exist. Using specific antibody enrichment followed by high-resolution mass spectrometry analysis, we identified hundreds of crotonylated proteins and lysine residues. Bioinformatics analysis reveals that crotonylated proteins are particularly enriched for nuclear proteins involved in RNA processing, nucleic acid metabolism, chromosome organization, and gene expression. Furthermore, we demonstrate that crotonylation regulates HDAC1 activity, expels HP1α from heterochromatin, and inhibits cell cycle progression through S-phase. Our data thus indicate that lysine crotonylation could occur in a large number of proteins and could have important regulatory roles in multiple nuclei-related cellular processes. |
| تدمد: | 1535-3907 1535-3893 |
| DOI: | 10.1021/acs.jproteome.7b00012 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b8f6de79bd17918870b848f942a1ef69 https://doi.org/10.1021/acs.jproteome.7b00012 |
| رقم الانضمام: | edsair.doi.dedup.....b8f6de79bd17918870b848f942a1ef69 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15353907 15353893 |
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| DOI: | 10.1021/acs.jproteome.7b00012 |