Early pregnancy-induced transcripts in peripheral blood immune cells in Bos indicus heifers
| العنوان: | Early pregnancy-induced transcripts in peripheral blood immune cells in Bos indicus heifers |
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| المؤلفون: | Sónia C. S. Andrade, Guilherme Pugliesi, Cecilia Constantino Rocha, Gabriela Dalmaso de Melo, Igor Garcia Motta, Mario Binelli, Luiz Lehmann Coutinho, Angela Maria Gonella-Diaza |
| المصدر: | Scientific Reports, Vol 10, Iss 1, Pp 1-15 (2020) Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP Scientific Reports |
| بيانات النشر: | Nature Publishing Group, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Transcription, Genetic, lcsh:Medicine, Early pregnancy factor, Biology, Beef cattle, Peripheral blood mononuclear cell, Article, Andrology, 03 medical and health sciences, 0302 clinical medicine, Immune system, Pregnancy, Transcription (biology), Animal physiology, medicine, Animals, Transcriptomics, lcsh:Science, Gene, Retrospective Studies, Multidisciplinary, lcsh:R, PRENHEZ, medicine.disease, Peripheral blood, 030104 developmental biology, Leukocytes, Mononuclear, biology.protein, Cattle, Female, lcsh:Q, 030217 neurology & neurosurgery |
| الوصف: | Immune cells play a central role in early pregnancy establishment in cattle. We aimed to: (1) discover novel early-pregnancy-induced genes in peripheral blood mononuclear cells (PBMC); and (2) characterize the temporal pattern of early-pregnancy-induced transcription of select genes in PBMC and peripheral blood polymorphonuclear cells (PMN). Beef heifers were artificially inseminated on D0 and pregnancies were diagnosed on D28. On D10, 14, 16, 18, and 20, blood was collected for isolation of PBMC and PMN from heifers that were retrospectively classified as pregnant (P) or non-pregnant (NP). PBMC samples from D18 were submitted to RNAseq and 220 genes were differentially expressed between pregnant (P) and non-pregnant (NP) heifers. The temporal abundance of 20 transcripts was compared between P and NP, both in PBMC and PMN. In PBMC, pregnancy stimulated transcription of IFI6, RSAD2, IFI44, IFITM2, CLEC3B, OAS2, TNFSF13B, DMKN and LGALS3BP as early as D18. Expression of IFI44, RSAD2, OAS2, LGALS3BP, IFI6 and C1R in PMN was stimulated in the P group from D18. The novel early-pregnancy induced genes discovered in beef heifers will allow both the understanding of the role of immune cells during the pre-attachment period and the development of technologies to detect early pregnancies in beef cattle. |
| اللغة: | English |
| تدمد: | 2045-2322 |
| DOI: | 10.1038/s41598-020-70616-8 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b8ba264902c87db1dc11471ef94615f4 http://link.springer.com/article/10.1038/s41598-020-70616-8 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....b8ba264902c87db1dc11471ef94615f4 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20452322 |
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| DOI: | 10.1038/s41598-020-70616-8 |