Development and evaluation of robust molecular markers linked to disease resistance in tomato for distinctness, uniformity and stability testing
| العنوان: | Development and evaluation of robust molecular markers linked to disease resistance in tomato for distinctness, uniformity and stability testing |
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| المؤلفون: | Ben Vosman, Laetitia Cavellini, Carmen Mansilla, André Moretti, Carole Caranta, Daniël Deinum, Hanneke van der Schoot, Paul Arens, René Mathis, Cécile Collonnier, Diederik Smilde, David Calvache, Sophie Rolland, Fernando Ponz |
| المساهمون: | Wageningen University and Research Centre (WUR), Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria = National Institute for Agricultural and Food Research and Technology (INIA), Groupe d'études et de contrôle des variétés et des semences, Institut National de la Recherche Agronomique (INRA), Génétique et Amélioration des Fruits et Légumes (GAFL), 1314 Gip Geves Direction - Siège, Institut National de la Recherche Agronomique (INRA)-Gip Geves Direction - Siège (Gip Geves Direction - Siège)-Accueil GEVES (Accueil GEVES)-Groupe d'étude et de controle des variétés et des semences (GEVES) |
| المصدر: | Theoretical and Applied Genetics, 120(3), 655-664 TAG Theoretical and Applied Genetics TAG Theoretical and Applied Genetics, Springer Verlag, 2010, 120 (3), pp.655-664. ⟨10.1007/s00122-009-1183-2⟩ Repositorio de Resultados de Investigación del INIA Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria INIA Theoretical and Applied Genetics 3 (120), 655-664. (2010) TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik INIA: Repositorio de Resultados de Investigación del INIA Theoretical and Applied Genetics 120 (2010) 3 |
| بيانات النشر: | Springer Nature |
| مصطلحات موضوعية: | [SDV.SA]Life Sciences [q-bio]/Agricultural sciences, 0106 biological sciences, family, Genetic Linkage, champignon phytopathogène, 01 natural sciences, tomate, chemistry.chemical_compound, Solanum lycopersicum, PRI Biodiversiteit en Veredeling, TOMATO MOSAIC VIRUS, Molecular marker, DISEASE RESISTANCE, Tomato mosaic virus, genes, database, nématode, 2. Zero hunger, Genetics, 0303 health sciences, MOLECULAR MARKERS, VERTICILLIUM DAHLIAE, MELOIDOGYNE INCOGNITA, GENETIQUE, nucleotide-binding, food and beverages, General Medicine, Physical Chromosome Mapping, gène de résistance, Agricultural sciences, dissection, varieties, lycopersicon esculentum, marqueur moléculaire, sélection végétale, Biotechnology, Genetic Markers, résistance aux maladies, construction, medicine.medical_specialty, Locus (genetics), virus, Plant disease resistance, Biology, Genes, Plant, 03 medical and health sciences, pcr, Molecular genetics, medicine, Plant breeding, Selection, Genetic, Gene, Plant Diseases, 030304 developmental biology, Original Paper, virus phytopathogène, locus, Reproducibility of Results, biology.organism_classification, Immunity, Innate, tm-2(2), PRI Biodiversity and Breeding, chemistry, Genetic Loci, Genetic marker, plante légumière, network, Agronomy and Crop Science, Sciences agricoles, 010606 plant biology & botany |
| الوصف: | Molecular markers linked to phenotypically important traits are of great interest especially when traits are difficult and/or costly to be observed. In tomato where a strong focus on resistance breeding has led to the introgression of several resistance genes, resistance traits have become important characteristics in distinctness, uniformity and stability (DUS) testing for Plant Breeders Rights (PBR) applications. Evaluation of disease traits in biological assays is not always straightforward because assays are often influenced by environmental factors, and difficulties in scoring exist. In this study, we describe the development and/or evaluation of molecular marker assays for the Verticillium genes Ve1 and Ve2, the tomato mosaic virusTm1 (linked marker), the tomato mosaic virus Tm2 and Tm22 genes, the Meloidogyne incognita Mi1-2 gene, the Fusarium I (linked marker) and I2 loci, which are obligatory traits in PBR testing. The marker assays were evaluated for their robustness in a ring test and then evaluated in a set of varieties. Although in general, results between biological assays and marker assays gave highly correlated results, marker assays showed an advantage over biological tests in that the results were clearer, i.e., homozygote/heterozygote presence of the resistance gene can be detected and heterogeneity in seed lots can be identified readily. Within the UPOV framework for granting of PBR, the markers have the potential to fulfil the requirements needed for implementation in DUS testing of candidate varieties and could complement or may be an alternative to the pathogenesis tests that are carried out at present. Electronic supplementary material The online version of this article (doi:10.1007/s00122-009-1183-2) contains supplementary material, which is available to authorized users. |
| وصف الملف: | application/octet-stream; application/pdf |
| اللغة: | English |
| تدمد: | 0040-5752 1432-2242 |
| DOI: | 10.1007/s00122-009-1183-2 |
| DOI: | 10.1007/s00122-009-1183-2⟩ |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b8561910cdbcd6b3a0d78b18cd959842 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....b8561910cdbcd6b3a0d78b18cd959842 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 00405752 14322242 |
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| DOI: | 10.1007/s00122-009-1183-2 |