Comprehensive lipidomic analysis of human plasma using multidimensional liquid- and gas-phase separations
| العنوان: | Comprehensive lipidomic analysis of human plasma using multidimensional liquid- and gas-phase separations |
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| المؤلفون: | Anna Baglai, Peter J. Schoenmakers, Ynze Mengerink, Sjoerd van der Wal, Maarten Honing, Andrea F.G. Gargano, Jan Jordens |
| المساهمون: | BioAnalytical Chemistry, AIMMS, Supramolecular Separations (HIMS, FNWI) |
| المصدر: | Baglai, A, Gargano, A F G, Jordens, J, Mengerink, Y, Honing, M, van der Wal, S & Schoenmakers, P J 2017, ' Comprehensive lipidomic analysis of human plasma using multidimensional liquid-and gas-phase separations : Two-dimensional liquid chromatography–mass spectrometry vs. liquid chromatography–trapped-ion-mobility–mass spectrometry ', Journal of Chromatography A, vol. 1530, pp. 90-103 . https://doi.org/10.1016/j.chroma.2017.11.014 Journal of Chromatography A, 1530, 90-103. Elsevier |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, Ion-mobility spectrometry, Analytical chemistry, Liquid phase, Mass spectrometry, 01 natural sciences, Biochemistry, Mass Spectrometry, Analytical Chemistry, Gas phase, 03 medical and health sciences, Liquid chromatography–mass spectrometry, Lipidomics, Humans, On-line comprehensive two-dimensional liquid chromatography, Chromatography, Untargeted analysis, Chemistry, 010401 analytical chemistry, Organic Chemistry, Trapped-ion-mobility–mass spectrometry, General Medicine, Lipids, 0104 chemical sciences, Characterization (materials science), 030104 developmental biology, Human plasma, Multidimensional separation, Blood Chemical Analysis, Chromatography, Liquid |
| الوصف: | Recent advancements in separation science have resulted in the commercialization of multidimensional separation systems that provide higher peak capacities and, hence, enable a more-detailed characterization of complex mixtures. In particular, two powerful analytical tools are increasingly used by analytical scientists, namely online comprehensive two-dimensional liquid chromatography (LC × LC, having a second-dimension separation in the liquid phase) and liquid chromatography-ion mobility-spectrometry (LC-IMS, second dimension separation in the gas phase). The goal of the current study was a general assessment of the liquid-chromatography–trapped-ion-mobility–mass spectrometry (LC-TIMS–MS) and comprehensive two-dimensional liquid chromatography–mass spectrometry (LC × LC–MS) platforms for untargeted lipid mapping in human plasma. For the first time trapped-ion-mobility spectrometry (TIMS) was employed for the separation of the major lipid classes and ion-mobility-derived collision-cross-section values were determined for a number of lipid standards. The general effects of a number of influencing parameters have been inspected and possible directions for improvements are discussed. We aimed to provide a general indication and practical guidelines for the analyst to choose an efficient multidimensional separation platform according to the particular requirements of the application. Analysis time, orthogonality, peak capacity, and an indicative measure for the resolving power are discussed as main characteristics for multidimensional separation systems. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 0021-9673 |
| DOI: | 10.1016/j.chroma.2017.11.014 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b4ec35388f42ace8c72fc292221961a1 https://hdl.handle.net/1871.1/003f5658-78f1-491b-bf87-2e4e5878c6cb |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....b4ec35388f42ace8c72fc292221961a1 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 00219673 |
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| DOI: | 10.1016/j.chroma.2017.11.014 |