Molecular epidemiology of Plasmodium falciparum resistance to antimalarial drugs in Indonesia
| العنوان: | Molecular epidemiology of Plasmodium falciparum resistance to antimalarial drugs in Indonesia |
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| المؤلفون: | Alan F. Cowman, Puji Budi Setia Asih, Hadya S. Nagesha, Gerard J. Casey, Jason D. Maguire, Din Syafruddin, John C. Reeder, J. Kevin Baird |
| المصدر: | Scopus-Elsevier |
| سنة النشر: | 2005 |
| مصطلحات موضوعية: | Genetics, biology, Molecular epidemiology, DHPS, Plasmodium falciparum, Drug resistance, biology.organism_classification, Virology, Infectious Diseases, Polymorphism (computer science), Dihydrofolate reductase, parasitic diseases, biology.protein, Parasitology, Restriction fragment length polymorphism, Dihydropteroate synthase |
| الوصف: | The extent of gene polymorphisms associated with resistance to chloroquine and sulfadoxine-pyrimethamine was examined in field isolates of Plasmodium falciparum from Indonesia. Eight malaria-endemic areas, representing a broad region of the western and eastern Indonesian Archipelago were surveyed. Blood from 20-50 patients was collected at each site, DNA was isolated, and the sequences of four different genes (dihydrofolate reductase [dhfr], dihydropteroate synthase [dhps], P. falciparum multidrug resistance 1 [pfmdr1], and P. falciparum chloroquine resistance transporter [pfcrt]) were analyzed using polymerase chain reaction and restriction fragment length polymorphisms to detect polymorphisms previously shown to be associated with resistance. This analysis identified polymorphisms in dhfr at 108-Asn/Thr, 16-Val, and 59-Arg. Polymorphisms in dhps were found less frequently, either 437-Gly alone or paired with 540-Glu. The pfcrt 76-Thr polymorphism was fixed in all parasite populations and pfmdr1 86-Tyr polymorphisms in all populations except in the most eastern regions. The pfmdr1 1042-Asp polymorphism occurred less frequently. These findings indicate that polymorphisms in genes associated with drug resistance in P. falciparum are found across a broad region of Indonesia. |
| اللغة: | English |
| تدمد: | 1476-1645 0002-9637 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b455f6a9f5bc5a0a8200c075482baacb http://ora.ox.ac.uk/objects/uuid:ede67a1d-466a-447f-9516-de9286b27cdb |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....b455f6a9f5bc5a0a8200c075482baacb |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14761645 00029637 |
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