التفاصيل البيبلوغرافية
| العنوان: |
β-Cell Loss and β-Cell Apoptosis in Human Type 2 Diabetes Are Related to Islet Amyloid Deposition |
| المؤلفون: |
Sakeneh Zraika, Steven E. Kahn, Per Westermark, Kathryn Aston-Mourney, Rebecca L. Hull, Mirna N. Toukatly, Corinne L. Fligner, Gunilla T. Westermark, Shoba L. Subramanian, Darcy B. Carr, Catherine A. Jurgens, Jayalakshmi Udayasankar |
| المصدر: |
The American Journal of Pathology. 178(6):2632-2640 |
| بيانات النشر: |
Elsevier BV, 2011. |
| سنة النشر: |
2011 |
| مصطلحات موضوعية: |
Adult, Male, Amyloid, Programmed cell death, medicine.medical_specialty, medicine.medical_treatment, Apoptosis, 030209 endocrinology & metabolism, Type 2 diabetes, Biology, Pathology and Forensic Medicine, Young Adult, 03 medical and health sciences, 0302 clinical medicine, Insulin-Secreting Cells, Internal medicine, Diabetes mellitus, mental disorders, medicine, Humans, Aged, Demography, 030304 developmental biology, Aged, 80 and over, 0303 health sciences, geography, TUNEL assay, geography.geographical_feature_category, Insulin, Regular Article, Middle Aged, Islet, medicine.disease, Endocrinology, Diabetes Mellitus, Type 2, Case-Control Studies, Female |
| الوصف: |
Amyloid deposition and reduced β-cell mass are pathological hallmarks of the pancreatic islet in type 2 diabetes; however, whether the extent of amyloid deposition is associated with decreased β-cell mass is debated. We investigated the possible relationship and, for the first time, determined whether increased islet amyloid and/or decreased β-cell area quantified on histological sections is correlated with increased β-cell apoptosis. Formalin-fixed, paraffin-embedded human pancreas sections from subjects with (n = 29) and without (n = 39) diabetes were obtained at autopsy (64 ± 2 and 70 ± 4 islets/subject, respectively). Amyloid and β cells were visualized by thioflavin S and insulin immunolabeling. Apoptotic β cells were detected by colabeling for insulin and by TUNEL. Diabetes was associated with increased amyloid deposition, decreased β-cell area, and increased β-cell apoptosis, as expected. There was a strong inverse correlation between β-cell area and amyloid deposition (r = −0.42, P < 0.001). β-Cell area was selectively reduced in individual amyloid-containing islets from diabetic subjects, compared with control subjects, but amyloid-free islets had β-cell area equivalent to islets from control subjects. Increased amyloid deposition was associated with β-cell apoptosis (r = 0.56, P < 0.01). Thus, islet amyloid is associated with decreased β-cell area and increased β-cell apoptosis, suggesting that islet amyloid deposition contributes to the decreased β-cell mass that characterizes type 2 diabetes. |
| تدمد: |
0002-9440 |
| DOI: |
10.1016/j.ajpath.2011.02.036 |
| URL الوصول: |
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b42a15e52317dd8c825283514747819e |
| Rights: |
OPEN |
| رقم الانضمام: |
edsair.doi.dedup.....b42a15e52317dd8c825283514747819e |
| قاعدة البيانات: |
OpenAIRE |