Quorum sensing network in clinical strains of A. baumannii: AidA is a new quorum quenching enzyme
| العنوان: | Quorum sensing network in clinical strains of A. baumannii: AidA is a new quorum quenching enzyme |
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| المؤلفون: | Nuria Tormo, María López Díaz, Jesús Rodríguez-Baño, Luis Anibarro, Lucía Blasco, Maria Tomas, Celia Mayer, Álvaro Pascual Hernández, Simona Mihaela Iftimie, Jordi Vila, German Bou, Ana Otero, Francisco Javier Casas-Ciria, Jerónimo Pachón, Andrea Muras, María de los Angeles Pallarés González, María Pilar Alonso García, Juan Pablo Horcajada, Laura Fernandez-Garcia, María de la Luz Cádiz-Gurrea, José Miguel Cisneros, Federico M. Ruiz, Azucena Rodriguez-Guardado |
| المساهمون: | Universidade de Santiago de Compostela. Departamento de Microbioloxía e Parasitoloxía, Instituto de Salud Carlos III, European Commission |
| المصدر: | Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela instname Minerva: Repositorio Institucional de la Universidad de Santiago de Compostela Universidad de Santiago de Compostela (USC) Digital.CSIC. Repositorio Institucional del CSIC PLoS ONE PLoS ONE, Vol 12, Iss 3, p e0174454 (2017) |
| بيانات النشر: | PLOS, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, Acinetobacter baumannii, Hydrolases, lcsh:Medicine, Pathology and Laboratory Medicine, Biochemistry, 4-Butyrolactone, Microbial Physiology, Medicine and Health Sciences, lcsh:Science, Pathogen, AidA, Cross Infection, Multidisciplinary, biology, Acinetobacter, Quorum Sensing, Clinical strains, Bacterial Pathogens, Enzymes, Quenching enzyme, Quorum Quenching, Medical Microbiology, Hyperexpression Techniques, Pathogens, A. baumannii, Research Article, Pathogen Motility, Virulence Factors, 030106 microbiology, Down-Regulation, Research and Analysis Methods, Microbiology, Bacterial genetics, 03 medical and health sciences, Extraction techniques, Bacterial Proteins, Intensive care, Escherichia coli, Homoserine, Gene Expression and Vector Techniques, Molecular Biology Techniques, Gene, Microbial Pathogens, Molecular Biology, Molecular Biology Assays and Analysis Techniques, Bacteria, lcsh:R, Organisms, Biology and Life Sciences, Proteins, Bacteriology, Hydrolase Gene, biology.organism_classification, RNA extraction, Quorum sensing, Genes, Bacterial, Biofilms, Enzymology, lcsh:Q, Bacterial Biofilms, Quorum |
| الوصف: | 18 p.-4 fig.-6 tab. López, María et al. Acinetobacter baumannii is an important pathogen that causes nosocomial infections generally associated with high mortality and morbidity in Intensive Care Units (ICUs). Currently, little is known about the Quorum Sensing (QS)/Quorum Quenching (QQ) systems of this pathogen. We analyzed these mechanisms in seven clinical isolates of A. baumannii. Microarray analysis of one of these clinical isolates, Ab1 (A. baumannii ST-2_clon_2010), previously cultured in the presence of 3-oxo-C12-HSL (a QS signalling molecule) revealed a putative QQ enzyme (α/ß hydrolase gene, AidA). This QQ enzyme was present in all non-motile clinical isolates (67% of which were isolated from the respiratory tract) cultured in nutrient depleted LB medium. Interestingly, this gene was not located in the genome of the only motile clinical strain growing in this medium (A. baumannii strain Ab421_GEIH-2010 [Ab7], isolated from a blood sample). The AidA protein expressed in E. coli showed QQ activity. Finally, we observed downregulation of the AidA protein (QQ system attenuation) in the presence of H2O2 (ROS stress). In conclusion, most of the A. baumannii clinical strains were not surface motile (84%) and were of respiratory origin (67%). Only the pilT gene was involved in surface motility and related to the QS system. Finally, a new QQ enzyme (α/ß hydrolase gene, AidA protein) was detected in these strains. This study was funded by grant PI13/02390 awarded to MT within the State Plan for R+D+I 2013-2016 (National Plan for Scientific Research, Technological Development and Innovation 2008-2011) and co-financed by the ISCIII-Deputy General Directorate of evaluation and Promotion of Research - European Regional Development Fund "A way of Making Europe" and Instituto de Salud Carlos III FEDER, Spanish Network for the Research in Infectious Diseases (REIPI RD12/0015). |
| وصف الملف: | application/pdf |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b41cd9d32a1c90803280bcc9cde6e26d http://hdl.handle.net/10347/23117 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....b41cd9d32a1c90803280bcc9cde6e26d |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |